Anti-Blooms Syndrome蛋白Blm抗体(ab5446)
Key features and details
- Goat polyclonal to Blooms Syndrome Protein Blm
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Blooms Syndrome蛋白Blm抗体
参阅全部 Blooms Syndrome Protein Blm 一抗 -
描述
山羊多克隆抗体to Blooms Syndrome蛋白Blm -
宿主
Goat -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee
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免疫原
Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.
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阳性对照
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/10000 - 1/25000. Predicted molecular weight: 159 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/10000 - 1/25000. Predicted molecular weight: 159 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. -
疾病相关
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability. -
序列相似性
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
翻译后修饰
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
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别名
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
图片
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab5446) at 0.04 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 159 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)Blooms Syndrome Protein Blm was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab5446 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab5446 at 1 µg/ml.
Lane 1: ab5446 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab5446 被引用在 2 文献中.
- Mooser C et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
- Leimbacher PA et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell 74:571-583.e8 (2019). PubMed: 30898438