Key features and details
- Goat polyclonal to Blooms Syndrome Protein Blm
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
参阅全部 Blooms Syndrome Protein Blm 一抗
描述山羊多克隆抗体to Blooms Syndrome蛋白Blm
经测试应用适用于: WB, IPmore details
Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
纯度Immunogen affinity purified
功能Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
疾病相关Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
序列相似性Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
翻译后修饰Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
- Information by UniProt
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab5446) at 0.04 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 159 kDa
Exposure time: 30 seconds
Blooms Syndrome Protein Blm was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab5446 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab5446 at 1 µg/ml.
Lane 1: ab5446 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
ab5446 被引用在 2 文献中.
- Mooser C et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
- Leimbacher PA et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell 74:571-583.e8 (2019). PubMed: 30898438