Anti-Blooms Syndrome蛋白Blm抗体(ab5446)

Reviews (3)Q&A (1)References (2)
Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
  • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

Key features and details

  • Goat polyclonal to Blooms Syndrome Protein Blm
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Blooms Syndrome蛋白Blm抗体
    参阅全部 Blooms Syndrome Protein Blm 一抗

  • 描述

    山羊多克隆抗体to Blooms Syndrome蛋白Blm

  • 宿主

    Goat

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Chimpanzee

  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.

  • 阳性对照

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5446于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/10000 - 1/25000. Predicted molecular weight: 159 kDa.
IP
Use at 2-10 µg/mg of lysate.
说明
WB
1/10000 - 1/25000. Predicted molecular weight: 159 kDa.
IP
Use at 2-10 µg/mg of lysate.

靶标

  • 功能

    Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.

  • 疾病相关

    Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.

  • 序列相似性

    Belongs to the helicase family. RecQ subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HRDC domain.

  • 翻译后修饰

    Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Blm antibody
      • BLM_HUMAN antibody
      • Bloom syndrome antibody
      • Bloom syndrome protein antibody
      • Bloom syndrome RecQ helicase like antibody
      • BS antibody
      • DNA helicase antibody
      • DNA helicase RecQ like type 2 antibody
      • MGC126616 antibody
      • MGC131618 antibody
      • MGC131620 antibody
      • RECQ 2 antibody
      • RECQ like antibody
      • RecQ like type 2 antibody
      • RecQ protein like 3 antibody
      • RecQ protein-like 3 antibody
      • RecQ-like type 2 antibody
      • RecQ2 antibody
      • RECQL 2 antibody
      • RECQL 3 antibody
      • RECQL2 antibody
      • RECQL3 antibody
      • type 2 antibody
      see all

    图片

    • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
      Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
      All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab5446) at 0.04 µg/ml

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 159 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
      Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

      Blooms Syndrome Protein Blm was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab5446 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab5446 at 1 µg/ml. 

      Lane 1: ab5446 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

    文献 (2)

    发表研究结果有使用 ab5446？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab5446 被引用在 2 文献中.

    • Mooser C  et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
    • Leimbacher PA  et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell 74:571-583.e8 (2019). PubMed: 30898438

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (HAP1)
    Gel Running Conditions
    Reduced Denaturing (6%)
    Loading amount
    20 µg
    Specification
    HAP1
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 23 2019

    Western blot abreview for Anti-Blooms Syndrome Protein Blm antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Reduced Denaturing (7% Tris-Bicine)
    Loading amount
    50 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 25 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Blooms Syndrome Protein Blm antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    DR. Kirk Mcmanus

    Verified customer

    提交于 Sep 30 2016

    Question

    ab2179 (Blooms Syndrome Protein Blm antibody) ab5446 (Blooms Syndrome Protein Blm antibody) Can you provide the immunogenic peptide sequence?

    Read More
    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab2179 and ab5446. Although they can not provide the exact immunogenic peptide sequence (proprietary), they can provide the following information: ab2179 - The epitope maps to a region between residue 1425 and the C-terminus (residue 1477) of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). ab5446 - The epitope maps to a region somewhere between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). I hope this information helps.

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