- 数据表
- 具体的参考文献 (28)
- 实验方案
概述
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产品名称Anti-Blooms Syndrome蛋白Blm抗体
参阅全部 Blooms Syndrome Protein Blm 一抗 -
描述兔多克隆抗体to Blooms Syndrome蛋白Blm
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宿主Rabbit
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经测试应用适用于: WB, IP, ICCmore details
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种属反应性与反应: Human
预测可用于: Mammals
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免疫原
His-tagged 376 amino acid C-terminal of human blm fusion protein.
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阳性对照
- HeLa nuclear extract.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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纯度Whole antiserum
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000. Detects a band of approximately 170 kDa (predicted molecular weight: 159 kDa). We have conflicting reports from customers about whether this antibody works in IF in HeLa or SK-N-SH cells. We would appreciate any customer feedback about this antibody. | |
| IP | Use at an assay dependent dilution. | |
| ICC | Use at an assay dependent dilution. |
靶标
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功能Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
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疾病相关Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
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序列相似性Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain. -
翻译后修饰Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
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细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 725208 Human
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别名
- Blm antibody
- BLM_HUMAN antibody
- Bloom syndrome antibody
see all
图片
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Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab476)Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band.
Western blot using ab476 against HELA nuclear extract revealing the 160kD Blm protein which, in gels, moves as a 190kD band. -
Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab476)Image from S Raynard et al, J Biol Chem 281:13861-4 (2006), Fig 2.BLAP75 physically interacts with BLM and Topo IIIa. Purified BLM was incubated with purified Topo IIIa and the reaction mixture was subjected to Immunoprecipitation with ab476. The reaction supernatant (S), wash (W), and eluate (E) were analyzed by SDS-PAGE, GST-BLAP75 or GST alone was incubated with BLM. Protein complexes were captured on glutathione-Sepharose beads, followed by SDS-PAGE.
实验方案
文献
This product has been referenced in:
- Hampp S et al. DNA damage tolerance pathway involving DNA polymerase ? and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A 113:E4311-9 (2016). WB ; Human . Read more (PubMed: 27407148) »
- Yang Y et al. Arginine methylation facilitates the recruitment of TOP3B to chromatin to prevent R loop accumulation. Mol Cell 53:484-97 (2014). Read more (PubMed: 24507716) »
客户评价及客户问答
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