Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (8%)
Sample
Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells (TRex CHO ))
Specification
Chinese Hamster Ovary cells (TRex CHO )
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk TBS-Tween
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/6000
Detection
Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Positive control
Whole cell extracts from human colon carcinoma cells
Additional data
Additional Notes
It doesn't work in Hamster Cells.
Abcam response
This antibody was tested in hamster using our AbTrial program. Although it appears that with these experimental conditions the antibody may not work in hamster, the customer did receive their AbTrial discount code which can be used on their next purchase.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Eleni Petsalaki
Verified customer
提交于 Feb 05 2014