Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse testis)
Loading amount
100 µg
Specification
mouse testis
Treatment
Irradiation - 20 gy
Gel Running Conditions
Reduced Denaturing (8% Precast gel (Invitrogen))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 160 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 12 2012