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Cardiovascular Blood Blood Cell Antigens

Anti-Blood Group Lewis b抗体[2-25LE] (ab3968)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (16)

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Key features and details

  • Mouse monoclonal [2-25LE] to Blood Group Lewis b
  • Reacts with: Human
  • Isotype: IgG1

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Anti-Blood Group Lewis a antibody [7LE] (ab3967)

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概述

  • 产品名称

    Anti-Blood Group Lewis b抗体[2-25LE]
  • 描述

    小鼠单克隆抗体[2-25LE] to Blood Group Lewis b
  • 宿主

    Mouse
  • 种属反应性

    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
  • 克隆

    单克隆
  • 克隆编号

    2-25LE
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    unknown
  • 研究领域

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

靶标

  • 相关性

    Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.
  • 细胞定位

    Plasma membrane - adsorbed onto the surface of erythrocytes.
  • 数据库链接

    • Entrez Gene: 4586 Human
    • SwissProt: P98088 Human
    • Unigene: 534332 Human
    • Unigene: 558950 Human
    • Unigene: 721515 Human
    • 别名

      • gastric mucin antibody
      • leB antibody
      • lewis antigen system antibody
      • lewis b antibody
      • lewis B blood group antigen antibody
      • lewisb antibody
      • major airway glycoprotein antibody
      • MUC5 antibody
      • mucin 5, subtypes A and C, tracheobronchial/gastric antibody
      • mucin 5AC, oligomeric mucus/gel-forming antibody
      • mucin 5AC, oligomeric mucus/gel-forming pseudogene antibody
      • mucin-5 subtype AC, tracheobronchial antibody
      • TBM antibody
      • tracheobronchial mucin antibody
      see all

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (16)

    发表研究结果有使用 ab3968?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3968 被引用在 16 文献中.

    • Almand EA  et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
    • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
    • Cuello HA  et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget 9:34176-34188 (2018). PubMed: 30344930
    • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
    • Yu Y  et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    I have ordered a mouse monoclonal [2-25LE] to blood group Lewis B (ab3968) and plan to perform the immunoprecipitation to capture the specific proteins which reacted with this antibody. I would like to inquire whether this antibody is available to do IP. Thanks a lot.

    Read More

    Abcam community

    Verified customer

    Asked on May 04 2012

    Answer

    Thank you for contacting us.

    We have not tested the antibody ab3968 for immunoprecipitation, and we have not received reports from any customers who have tried it for IP. It is possible it will be useful for IP but we have no data that confirms this.

    We have one other antibody against this target, blood group Lewis B, also monoclonal, but it is untested for IP. Here is a URL link to the datasheet:

    https://www.abcam.com/Blood-Group-Lewis-b-antibody-T218-ab3357.html

    For IP with monoclonal antibodies,a better yield and greater specificity can be obtained by mixing monoclonal antibodies raised against the same protein and then adding the mixed antibodies to the sample, rather than trying to IP with a single monoclonal antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 May 04 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Blood Group Lewis b antibody [2-25LE]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human colorectal cell line C170)
    Specification
    Human colorectal cell line C170
    Fixative
    Formaldehyde
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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