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Anti-Blood Group Lewis b抗体[2-25LE] (ab3968)

Reviews (1)Q&A (1)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

    Key features and details

    • Mouse monoclonal [2-25LE] to Blood Group Lewis b
    • Suitable for: WB, ELISA, Flow Cyt, ICC/IF, IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Blood Group Lewis b抗体[2-25LE]

    • 描述

      小鼠单克隆抗体[2-25LE] to Blood Group Lewis b

    • 宿主

      Mouse

    • 经测试应用

      适用于: WB, ELISA, Flow Cyt, ICC/IF, IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Full length native protein (purified) corresponding to Human Blood Group Lewis b. Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

    • 阳性对照

      • IHC: Human colon adenocarcinoma

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 纯度

      Protein A purified

    • 纯化说明

      Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

    • 克隆

      单克隆

    • 克隆编号

      2-25LE

    • 骨髓瘤

      unknown

    • 同种型

      IgG1

    • 轻链类型

      unknown

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3968于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 23144050

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    ICC/IF (1)
    Use at an assay dependent concentration. PubMed: 17202152
    IHC-P
    Use a concentration of 5 - 10 µg/ml.
    说明
    WB
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 23144050

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 17202152
    IHC-P
    Use a concentration of 5 - 10 µg/ml.

    靶标

    • 相关性

      Blood group Lewis b is a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals.

    • 细胞定位

      Plasma membrane - adsorbed onto the surface of erythrocytes.

    • 数据库链接

      • 别名

        • gastric mucin antibody
        • leB antibody
        • lewis antigen system antibody
        • lewis b antibody
        • lewis B blood group antigen antibody
        • lewisb antibody
        • major airway glycoprotein antibody
        • MUC5 antibody
        • mucin 5, subtypes A and C, tracheobronchial/gastric antibody
        • mucin 5AC, oligomeric mucus/gel-forming antibody
        • mucin 5AC, oligomeric mucus/gel-forming pseudogene antibody
        • mucin-5 subtype AC, tracheobronchial antibody
        • TBM antibody
        • tracheobronchial mucin antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon adenocarcinoma tissue labelling Blood Group Lewis b with ab3968 at 10 µg/mL.

      文献 (16)

      发表研究结果有使用 ab3968？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3968 被引用在 16 文献中.

      • Almand EA  et al. Characterization of human norovirus binding to gut-associated bacterial ligands. BMC Res Notes 12:607 (2019). PubMed: 31547886
      • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
      • Cuello HA  et al. Aberrant O-glycosylation modulates aggressiveness in neuroblastoma. Oncotarget 9:34176-34188 (2018). PubMed: 30344930
      • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
      • Yu Y  et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-2 of 2 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Blood Group Lewis b antibody [2-25LE]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Human colorectal cell line C170)
      Specification
      Human colorectal cell line C170
      Fixative
      Formaldehyde
      Permeabilization
      No
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 09 2011

      Question

      I have ordered a mouse monoclonal [2-25LE] to blood group Lewis B (ab3968) and plan to perform the immunoprecipitation to capture the specific proteins which reacted with this antibody. I would like to inquire whether this antibody is available to do IP. Thanks a lot.

      Read More
      Answer

      Thank you for contacting us.

      We have not tested the antibody ab3968 for immunoprecipitation, and we have not received reports from any customers who have tried it for IP. It is possible it will be useful for IP but we have no data that confirms this.

      We have one other antibody against this target, blood group Lewis B, also monoclonal, but it is untested for IP. Here is a URL link to the datasheet:

      https://www.abcam.com/Blood-Group-Lewis-b-antibody-T218-ab3357.html

      For IP with monoclonal antibodies,a better yield and greater specificity can be obtained by mixing monoclonal antibodies raised against the same protein and then adding the mixed antibodies to the sample, rather than trying to IP with a single monoclonal antibody.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com