Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal carcinoma)
Specification
Colorectal carcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Universal Elite Vectorstain ABC kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
After heat mediated antigen retrieval in citrate buffer, pH 6.0, 20 mins, peroxidase activity was blocked before blocking with normal serum and avidin biotin block.The primary antibody was dilulted to 1:400 and incubated for 1 hour at room temperature. Vectorstain Universal ABC kit used. DAB was used as the chromagen and slide was counterstained with haematoxylin.
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提交于 Mar 02 2011