Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestine)
Loading amount
50 µg
Specification
Intestine
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA, 1 x TBS, 0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: HRP conjugated goat anti mouse
Host species: Mouse
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Mouse
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/10000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 25, 27, 30, 50, 60, kDa Non-specific: 25, 37, 100, 150, 250 kDa
Additional data
Additional Notes
Primary antibody was used at 1 in 1000 dilution and showed up many of the bands in the marker lane as non specific bands. Diluting the antibody further should reduce or eliminate the presence of these bands
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 19 2010