为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Cardiovascular Blood Blood Cell Antigens

Anti-Blood Group Lewis a抗体[7LE] (ab3967)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (8)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)

    Key features and details

    • Mouse monoclonal [7LE] to Blood Group Lewis a
    • Suitable for: IHC-P
    • Reacts with: Mouse, Human
    • Isotype: IgG1

    你可能会感兴趣

    一抗
    Product image
    Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)
    二抗
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    一抗
    Product image
    Anti-Blood Group Lewis b antibody [2-25LE] (ab3968)

    查看更多相关产品

    概述

    • 产品名称

      Anti-Blood Group Lewis a抗体[7LE]
      参阅全部 Blood Group Lewis a 一抗
    • 描述

      小鼠单克隆抗体[7LE] to Blood Group Lewis a
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Mouse, Human
    • 免疫原

      Full length native protein (purified) corresponding to Human Blood Group Lewis a. Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 纯化说明

      Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
    • 克隆

      单克隆
    • 克隆编号

      7LE
    • 骨髓瘤

      unknown
    • 同种型

      IgG1
    • 轻链类型

      unknown
    • 研究领域

      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • RBC Antigens
      • Blood Group Antigens

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3967于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 相关性

      Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
    • 细胞定位

      Cytoplasmic
    • 别名

      • Lewis A antigen antibody

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)This image is courtesy of an Anonymous Abreview.
      ab3967 staining Blood Group Lewis a in Human colorectal carcinoma tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were PFA-fixed and subjected to heat-mediated antigen retrieval in citric acid (pH 6.0, citrate buffer) prior to blocking with 2% serum for 20 minutes at 22°C. The primary antibody was used undiluted and incubated with the sample for 1 hour at 22°C. An biotinylated universal ABC kit was used for detection.

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (8)

    发表研究结果有使用 ab3967?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3967 被引用在 8 文献中.

    • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
    • Tang H  et al. Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9. Cell Mol Gastroenterol Hepatol 2:201-221.e15 (2016). PubMed: 26998508
    • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
    • Yu Y  et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
    • Nyström K  et al. Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells. Glycobiology 17:355-66 (2007). PubMed: 17202152
    • Creuzot-Garcher C  et al. Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci 40:1631-6 (1999). PubMed: 10393028
    • Torrado J  et al. Lewis antigen alterations in gastric cancer precursors. Gastroenterology 102:424-30 (1992). PubMed: 1732113
    • Bara J  et al. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J 254:185-93 (1988). PubMed: 2460087

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Blood Group Lewis a antibody [7LE]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colorectal carcinoma)
    Specification
    Colorectal carcinoma
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 02 2011

    Flow Cytometry abreview for Anti-Blood Group Lewis a antibody [7LE]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Human colorectal cell line C170)
    Specification
    Human colorectal cell line C170
    Preparation
    Cell harvesting/tissue preparation method: Typsinisation
    Sample buffer: PBS
    Permeabilization
    No
    Gating Strategy
    Ungated
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 11 2011

    Western blot abreview for Anti-Blood Group Lewis a antibody [7LE]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestine)
    Loading amount
    50 µg
    Specification
    Intestine
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-20%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2022 艾博抗(上海)贸易有限公司版权所有