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Cardiovascular Blood Blood Cell Antigens

Anti-Blood Group Lewis a抗体[7LE] (ab3967)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (8)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)

    Key features and details

    • Mouse monoclonal [7LE] to Blood Group Lewis a
    • Suitable for: IHC-P
    • Reacts with: Mouse, Human
    • Isotype: IgG1

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    概述

    • 产品名称

      Anti-Blood Group Lewis a抗体[7LE]
      参阅全部 Blood Group Lewis a 一抗
    • 描述

      小鼠单克隆抗体[7LE] to Blood Group Lewis a
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Mouse, Human
    • 免疫原

      Full length native protein (purified) corresponding to Human Blood Group Lewis a. Mucin isolated by size-exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio.s classification).

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 纯化说明

      Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.
    • 克隆

      单克隆
    • 克隆编号

      7LE
    • 骨髓瘤

      unknown
    • 同种型

      IgG1
    • 轻链类型

      unknown
    • 研究领域

      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • RBC Antigens
      • Blood Group Antigens

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3967于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 相关性

      Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
    • 细胞定位

      Cytoplasmic
    • 别名

      • Lewis A antigen antibody

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis a antibody [7LE] (ab3967)This image is courtesy of an Anonymous Abreview.
      ab3967 staining Blood Group Lewis a in Human colorectal carcinoma tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were PFA-fixed and subjected to heat-mediated antigen retrieval in citric acid (pH 6.0, citrate buffer) prior to blocking with 2% serum for 20 minutes at 22°C. The primary antibody was used undiluted and incubated with the sample for 1 hour at 22°C. An biotinylated universal ABC kit was used for detection.

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (8)

    发表研究结果有使用 ab3967?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3967 被引用在 8 文献中.

    • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
    • Tang H  et al. Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9. Cell Mol Gastroenterol Hepatol 2:201-221.e15 (2016). PubMed: 26998508
    • Zhuang H  et al. Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells. Biochimie 105:91-8 (2014). Human . PubMed: 24998328
    • Yu Y  et al. Human milk contains novel glycans that are potential decoy receptors for neonatal rotaviruses. Mol Cell Proteomics 13:2944-60 (2014). PubMed: 25048705
    • Nyström K  et al. Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells. Glycobiology 17:355-66 (2007). PubMed: 17202152
    • Creuzot-Garcher C  et al. Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci 40:1631-6 (1999). PubMed: 10393028
    • Torrado J  et al. Lewis antigen alterations in gastric cancer precursors. Gastroenterology 102:424-30 (1992). PubMed: 1732113
    • Bara J  et al. Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J 254:185-93 (1988). PubMed: 2460087

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Blood Group Lewis a antibody [7LE]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colorectal carcinoma)
    Specification
    Colorectal carcinoma
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 02 2011

    Flow Cytometry abreview for Anti-Blood Group Lewis a antibody [7LE]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Human colorectal cell line C170)
    Specification
    Human colorectal cell line C170
    Preparation
    Cell harvesting/tissue preparation method: Typsinisation
    Sample buffer: PBS
    Permeabilization
    No
    Gating Strategy
    Ungated
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 11 2011

    Western blot abreview for Anti-Blood Group Lewis a antibody [7LE]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestine)
    Loading amount
    50 µg
    Specification
    Intestine
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-20%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 19 2010

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