概述

  • 产品名称
  • 描述
    兔多克隆抗体to BLNK
  • 宿主
    Rabbit
  • 特异性
    This antibody detects endogenous levels of total BLNK protein.
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthesized non-phosphopeptide derived from human BLNK around the phosphorylation site of tyrosine 96.

  • 阳性对照
    • Human tonsil tissue sections and extracts from 293 cells treated with etoposide.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab73706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
  • 组织特异性
    Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
  • 疾病相关
    Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • 序列相似性
    Contains 1 SH2 domain.
  • 翻译后修饰
    Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
  • 细胞定位
    Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGM4 antibody
    • B cell adapter containing SH2 domain protein antibody
    • B cell adapter containing Src homology 2 domain protein antibody
    • B cell adaptor containing SH2 domain antibody
    • B cell linker antibody
    • B cell linker protein antibody
    • B cell-specific adaptor protein antibody
    • B-cell activation antibody
    • B-cell adapter containing a SH2 domain protein antibody
    • B-cell adapter containing a Src homology 2 domain protein antibody
    • B-cell linker protein antibody
    • BASH antibody
    • Bca antibody
    • BLNK antibody
    • BLNK s antibody
    • BLNK_HUMAN antibody
    • Cytoplasmic adapter protein antibody
    • Ly 57 antibody
    • Ly57 antibody
    • Lymphocyte antigen 57 antibody
    • Lyw 57 antibody
    • Lyw57 antibody
    • MGC111051 antibody
    • SH2 domain-containing leukocyte protein, 65-KD antibody
    • SLP 65 antibody
    • SLP-65 antibody
    • SLP65 antibody
    • Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody
    • Src homology 2 domain containing leukocyte protein of 65 kDa antibody
    • Src homology 2 domain-containing leukocyte protein of 65 kDa antibody
    see all

图片

  • All lanes : Anti-BLNK antibody (ab73706) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, treated with etoposide (25µM, 24hours).
    Lane 2 : Extracts from 293 cells, treated with etoposide (25µM, 24hours) plus 5µg immunizing peptide.

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using ab73706 at a 1:50 dilution.

    Left image un-treated.

    Right image treated with immunizing peptide.

文献

ab73706 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human B lymphoma cells,Raji)
Loading amount
40 µg
Specification
Human B lymphoma cells,Raji
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Mar 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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