Anti-BLNK抗体(ab2230)
Key features and details
- Goat polyclonal to BLNK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BLNK抗体
参阅全部 BLNK 一抗 -
描述
山羊多克隆抗体to BLNK -
宿主
Goat -
Tested Applications & Species
Application Species WB Human -
免疫原
Synthetic peptide: KDSTRLKYAVKVS, corresponding to C terminal amino acids 444-456 of Human BLNK.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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WB |
Human
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All applications |
Mouse
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应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.
A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate. |
说明 |
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WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa. A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate. |
靶标
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功能
Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis. -
组织特异性
Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. -
疾病相关
Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
序列相似性
Contains 1 SH2 domain. -
翻译后修饰
Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways. -
细胞定位
Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction. - Information by UniProt
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数据库链接
- Entrez Gene: 29760 Human
- Entrez Gene: 17060 Mouse
- Omim: 604515 Human
- SwissProt: Q8WV28 Human
- SwissProt: Q9QUN3 Mouse
- Unigene: 665244 Human
- Unigene: 9749 Mouse
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别名
- AGM4 antibody
- B cell adapter containing SH2 domain protein antibody
- B cell adapter containing Src homology 2 domain protein antibody
see all
图片
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Anti-BLNK antibody (ab2230) at 4 µg/ml + Cell lysate prepared from human Daudi cells at 35 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Ab2230 staining (4µ g/ml) of Daudi lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence Primary incubated for 1 hour. -
All lanes : Anti-BLNK antibody (ab2230) at 1 µg/ml
Lane 1 : Cell lysate prepared from HEK293 mock transfected cells
Lane 2 : Cell lysate prepared from HEK293 overexpressing BLNK
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab2230 尚未被引用在任何文献中。