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Immunology Adaptive Immunity B Cells Non-CD

Anti-BLNK抗体(ab2230)

  • Datasheet
  • SDS
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Western blot - Anti-BLNK antibody (ab2230)
  • Western blot - Anti-BLNK antibody (ab2230)

Key features and details

  • Goat polyclonal to BLNK
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-BLNK抗体
    参阅全部 BLNK 一抗
  • 描述

    山羊多克隆抗体to BLNK
  • 宿主

    Goat
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide: KDSTRLKYAVKVS, corresponding to C terminal amino acids 444-456 of Human BLNK.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Signal Transduction
    • Adapters
    • Transmembrane

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human BLNK protein (ab104120)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
WB
Human
All applications
Mouse
应用 Ab评论 说明
WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.

A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate.

说明
WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.

A 1 hour primary incubation is recommended for this product. Approx 70kDa band seen in Daudi lysate.

靶标

  • 功能

    Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
  • 组织特异性

    Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
  • 疾病相关

    Defects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • 序列相似性

    Contains 1 SH2 domain.
  • 翻译后修饰

    Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
  • 细胞定位

    Cytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
  • Target information above from: UniProt accession Q8WV28 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 29760 Human
    • Entrez Gene: 17060 Mouse
    • Omim: 604515 Human
    • SwissProt: Q8WV28 Human
    • SwissProt: Q9QUN3 Mouse
    • Unigene: 665244 Human
    • Unigene: 9749 Mouse
    • 别名

      • AGM4 antibody
      • B cell adapter containing SH2 domain protein antibody
      • B cell adapter containing Src homology 2 domain protein antibody
      • B cell adaptor containing SH2 domain antibody
      • B cell linker antibody
      • B cell linker protein antibody
      • B cell-specific adaptor protein antibody
      • B-cell activation antibody
      • B-cell adapter containing a SH2 domain protein antibody
      • B-cell adapter containing a Src homology 2 domain protein antibody
      • B-cell linker protein antibody
      • BASH antibody
      • Bca antibody
      • BLNK antibody
      • BLNK s antibody
      • BLNK_HUMAN antibody
      • Cytoplasmic adapter protein antibody
      • Ly 57 antibody
      • Ly57 antibody
      • Lymphocyte antigen 57 antibody
      • Lyw 57 antibody
      • Lyw57 antibody
      • MGC111051 antibody
      • SH2 domain-containing leukocyte protein, 65-KD antibody
      • SLP 65 antibody
      • SLP-65 antibody
      • SLP65 antibody
      • Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody
      • Src homology 2 domain containing leukocyte protein of 65 kDa antibody
      • Src homology 2 domain-containing leukocyte protein of 65 kDa antibody
      see all

    图片

    • Western blot - Anti-BLNK antibody (ab2230)
      Western blot - Anti-BLNK antibody (ab2230)
      Anti-BLNK antibody (ab2230) at 4 µg/ml + Cell lysate prepared from human Daudi cells at 35 µg

      Developed using the ECL technique.

      Predicted band size: 51 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Ab2230 staining (4µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence Primary incubated for 1 hour.
    • Western blot - Anti-BLNK antibody (ab2230)
      Western blot - Anti-BLNK antibody (ab2230)
      All lanes : Anti-BLNK antibody (ab2230) at 1 µg/ml

      Lane 1 : Cell lysate prepared from HEK293 mock transfected cells
      Lane 2 : Cell lysate prepared from HEK293 overexpressing BLNK

      Predicted band size: 51 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab2230?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2230 尚未被引用在任何文献中。

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