Anti-BIRC6/APOLLON抗体(ab19609)
Key features and details
- Rabbit polyclonal to BIRC6/APOLLON
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-BIRC6/APOLLON抗体
参阅全部 BIRC6/APOLLON 一抗 -
描述
兔多克隆抗体to BIRC6/APOLLON -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to BIRC6/APOLLON (C terminal).
Database link: Q9NR09 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19609于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/5000 - 1/15000. Predicted molecular weight: 528 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/5000 - 1/15000. Predicted molecular weight: 528 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Protects cells from undergoing apoptosis. -
组织特异性
Expressed in brain cancer cells. -
序列相似性
In the C-terminal section; belongs to the ubiquitin-conjugating enzyme family.
Contains 1 BIR repeat. -
翻译后修饰
Ubiquitinated. Ubiquitination is mediated by the RNF41 E3 ligase and leads to proteasomal degradation, impairing inhibition of apoptosis. - Information by UniProt
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数据库链接
- Entrez Gene: 57448 Human
- Entrez Gene: 12211 Mouse
- Omim: 605638 Human
- SwissProt: Q9NR09 Human
- SwissProt: O88738 Mouse
- Unigene: 150107 Human
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别名
- Apollon antibody
- Apollon protein antibody
- Baculoviral IAP repeat containing 6 antibody
see all
图片
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All lanes : Anti-BIRC6/APOLLON antibody (ab19609) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK293T whole cell lysate at 50 µg
Lane 4 : NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 528 kDa
Exposure time: 3 seconds -
Immunoprecipitaction of HeLa cells with ab19609
Lane 1: ab19609 current lot
Lane 2: ab19609 previous lot
Lane 3: control IgG
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Immunohistochemistry analysis of formalin-fixed paraffin-embedded human breast carcinoma tissue staining BIRC6/Apollon with ab19609 at 0.2µg/ml
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab19609 被引用在 12 文献中.
- Wang D et al. Cold Atmospheric Plasma Conveys Selectivity Against Hepatocellular Carcinoma Cells via Triggering EGFR(Tyr1068)-Mediated Autophagy. Front Oncol 12:895106 (2022). PubMed: 35860596
- Tong F et al. Identification of an autophagy-related 12-lncRNA signature and evaluation of NFYC-AS1 as a pro-cancer factor in lung adenocarcinoma. Front Genet 13:834935 (2022). PubMed: 36105077
- Li Y et al. Overexpression of BIRC6 driven by EGF-JNK-HECTD1 signaling is a potential therapeutic target for triple-negative breast cancer. Mol Ther Nucleic Acids 26:798-812 (2021). PubMed: 34729249
- Mukherjee SP et al. Next-Generation Sequencing Reveals Differential Responses to Acute versus Long-Term Exposures to Graphene Oxide in Human Lung Cells. Small 16:e1907686 (2020). PubMed: 32227449
- Park JS et al. Alpha-lipoic acid attenuates p-cresyl sulfate-induced renal tubular injury through suppression of apoptosis and autophagy in human proximal tubular epithelial cells. Biomed Pharmacother 112:108679 (2019). PubMed: 30798133