Biotinylated山羊抗小鼠&兔IgG (H+L) (Ready To Use) (ab64257)
概述
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产品名称
Biotinylated山羊抗小鼠&兔IgG (H+L) (Ready To Use)
参阅全部 IgG 试剂 -
经测试应用
适用于: IHC-Pmore details -
常规说明
Staining protocol
- Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
- Add enough drops of an appropriate hydrogen peroxide block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
- Perform appropriate pretreatment if required. Wash 3 times in buffer.
- Apply an appropriate protein block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
- Apply primary antibody and incubate according to manufacturer's protocol.
- Wash 4 times in buffer. Apply Biotinylated Goat anti Mouse & Rabbit IgG(H+L) solution (ab64257) and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
- Apply enzyme labeled streptavidin and incubate according to manufacturer’s protocol.
- Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
- Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.
- Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
性能
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形式
Liquid -
存放说明
Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Reagent说明
This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen. -
研究领域
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细胞定位
Secreted -
别名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all -
细胞定位
Secreted
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64257于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent dilution.
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说明 |
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IHC-P
Use at an assay dependent dilution. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab64257 被引用在 10 文献中.
- Smith SM et al. Paired associates learning is disrupted after unilateral parietal lobe controlled cortical impact in rats: A trial-by-trial behavioral analysis. Behav Brain Res 437:114106 (2023). PubMed: 36089100
- Mota RF et al. RANKL Impairs the TLR4 Pathway by Increasing TRAF6 and RANK Interaction in Macrophages. Biomed Res Int 2022:7740079 (2022). PubMed: 35463988
- Zeidi M et al. Increased CD69+CCR7+ circulating activated T cells and STAT3 expression in cutaneous lupus erythematosus patients recalcitrant to antimalarials. Lupus 31:472-481 (2022). PubMed: 35258358
- Zeidi M et al. Increased MxA protein expression and dendritic cells in spongiotic dermatitis differentiates dermatomyositis from eczema in a single-center case-control study. J Cutan Pathol 48:364-373 (2021). PubMed: 32954523
- Chen KL et al. Myeloid Dendritic Cells Are Major Producers of IFN-β in Dermatomyositis and May Contribute to Hydroxychloroquine Refractoriness. J Invest Dermatol 141:1906-1914.e2 (2021). PubMed: 33675790
- Diaz-Lucena D et al. TREM2 expression in the brain and biological fluids in prion diseases. Acta Neuropathol 141:841-859 (2021). PubMed: 33881612
- Zhao QH et al. Matrix metalloproteinase-13, NF-?B p65 and interleukin-1ß are associated with the severity of knee osteoarthritis. Exp Ther Med 19:3620-3626 (2020). PubMed: 32346426
- Li R et al. Tree shrew as a new animal model to study the pathogenesis of avian influenza (H9N2) virus infection. Emerg Microbes Infect 7:166 (2018). PubMed: 30301950
- Xu H et al. Inhibitor ?Ba protein therapy alleviates severe pneumonia through inhibition of nuclear factor ?B. Exp Ther Med 13:1398-1402 (2017). PubMed: 28413484
- Fei B et al. Expression and clinical significance of Beclin-1 in gastric cancer tissues of various clinical stages. Oncol Lett 11:2271-2277 (2016). PubMed: 26998161