生物素Anti-VSV-G tag抗体(ab34774)
Key features and details
- Biotin Rabbit polyclonal to VSV-G tag
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-VSV-G tag抗体
参阅全部 VSV-G tag 一抗 -
描述
生物素兔多克隆抗体to VSV-G tag -
宿主
Rabbit -
偶联物
Biotin -
特异性
This antibody is directed against VSV-G protein. -
经测试应用
适用于: ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to VSV-G tag aa 501-511.
Sequence:YTDIEMNRLGK
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常规说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34774于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | (1) |
1/3000 - 1/12000.
|
| WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 57.5 kDa.
|
| ICC/IF | (2) |
1/200 - 1/1000. We recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein.
|
| 说明 |
|---|
|
ELISA
1/3000 - 1/12000. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 57.5 kDa. |
|
ICC/IF
1/200 - 1/1000. We recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. |
靶标
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相关性
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding. -
别名
- Vesicular stomatitis virus glycoprotein tag antibody
- VSV epitope tag antibody
- VSV tag antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-VSV-G tag antibody (ab34774)Image courtesy of an anonymous Abreview.ab34774 staining VSV-G tag in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.025% Triton-X in PBS and then blocked using 5% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat polyclonal conjugated to Alexa Fluor® 488 (green) used undiluted.
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Western blot - Biotin Anti-VSV-G tag antibody (ab34774)Image courtesy of an anonymous Abreview.Both lanes are ab34774 at a 1/500 dilution plus 20µg of whole cell lysate prepared from HeLa cells transfected with VSV-G. A Streptavidin HRP conjugate was used at a 1/2000 dilution.
Primary antibody incubated for 16 hours at 4°C.Blocking step performed using 5% milk for 1 hour at 23°C. -
ELISA - Biotin Anti-VSV-G tag antibody (ab34774)This image is courtesy of an anonymous Abreviewab34774 detecting the VSV-G tag in HeLa cells expressing VSV-G by direct ELISA. Samples were blocked with 5% BSA for 1 hour at 23°C and incubated with the primary antibody (1/3000 in blocking buffer) for 16 hours at 4°C. An undiluted streptavidin-alkaline phosphatase conjugate polyclonal was used as the secondary antibody.
实验方案
数据表及文件
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Datasheet download
文献 (6)
ab34774 被引用在 6 文献中.
- Kamps D et al. Optogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network. Cell Rep 33:108467 (2020). PubMed: 33264629
- Thompson A et al. Molecular Characterisation of Small Molecule Agonists Effect on the Human Glucagon Like Peptide-1 Receptor Internalisation. PLoS One 11:e0154229 (2016). PubMed: 27100083
- Thompson A & Kanamarlapudi V Distinct regions in the C-Terminus required for GLP-1R cell surface expression, activity and internalisation. Mol Cell Endocrinol 413:66-77 (2015). PubMed: 26116235
- Wan Z et al. Fangchinoline inhibits human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing. PLoS One 7:e39225 (2012). WB . PubMed: 22720080
- Fukuhara T et al. Intracellular delivery of serum-derived hepatitis C virus. Microbes Infect 13:405-12 (2011). PubMed: 21262370
- Tani H et al. Replication-competent recombinant vesicular stomatitis virus encoding hepatitis C virus envelope proteins. J Virol 81:8601-12 (2007). WB . PubMed: 17553880
