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Cardiovascular Blood Fibrinolysis / Thrombolysis

生物素Anti-Plasminogen抗体(ab7335)

  • Datasheet
Reviews (1)Q&A (1)References (1)

Product price, shipping and contact information

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Key features and details

  • Biotin Goat polyclonal to Plasminogen
  • Suitable for: IHC-Fr, Conjugation, Dot blot, Immunomicroscopy, Flow Cyt, ELISA, WB, ICC/IF
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

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概述

  • 产品名称

    生物素Anti-Plasminogen抗体
    参阅全部 Plasminogen 一抗
  • 描述

    生物素山羊多克隆抗体to Plasminogen
  • 宿主

    Goat
  • 偶联物

    Biotin
  • 经测试应用

    适用于: IHC-Fr, Conjugation, Dot blot, Immunomicroscopy, Flow Cyt, ELISA, WB, ICC/IFmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Plasminogen. Human plasma
    Database link: P00747

  • 常规说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 纯化说明

    IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Blood
    • Fibrinolysis / Thrombolysis
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic inhibitory factors
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases

相关产品

  • Isotype control

    • Biotin Goat IgG - Isotype Control (ab37376)
  • Recombinant Protein

    • Recombinant human Plasminogen protein (Active) (ab200264)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7335于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt (1)
Use at an assay dependent concentration.

ab37376 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA
Use at an assay dependent concentration.

As well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 19182206
说明
IHC-Fr
Use at an assay dependent concentration.
Conjugation
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab37376 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA
Use at an assay dependent concentration.

As well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 19182206

靶标

  • 功能

    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.
  • 组织特异性

    Present in plasma and many other extracellular fluids. It is synthesized in the liver.
  • 疾病相关

    Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
    Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa.
  • 序列相似性

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.
  • 结构域

    Kringle domains mediate interaction with CSPG4.
  • 翻译后修饰

    N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.
  • 细胞定位

    Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Target information above from: UniProt accession P00747 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5340 Human
    • Omim: 173350 Human
    • SwissProt: P00747 Human
    • Unigene: 143436 Human
    • 形式

      Cleaved into the following 5 chains: 1.Plasmin heavy chain A2.Activation peptide3.Angiostatin4.Plasmin heavy chain A, short form5. Plasmin light chain B
    • 别名

      • Plasmin antibody
      • Plasmin heavy chain A antibody
      • Plasmin light chain B antibody
      • Plasminogen antibody
      • PLG antibody
      • PLMN_HUMAN antibody
      see all

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab7335?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7335 被引用在 1 文献中.

    • Wygrecka M  et al. Enolase-1 promotes plasminogen-mediated recruitment of monocytes to the acutely inflamed lung. Blood 113:5588-98 (2009). WB, ICC/IF, IHC-Fr ; Human . PubMed: 19182206

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Plasminogen antibody (Biotin)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (muscle,rhabdomyosarcoma)
    Specification
    muscle,rhabdomyosarcoma
    Preparation
    Cell harvesting/tissue preparation method: the cell were trypsinized and counted to 5x10^5 cell/ each group
    Sample buffer: FACS buffer
    Fixation
    No
    Permeabilization
    No
    Gating Strategy
    the cells were gated on FSC/SSC to remove cell debris
    Read More

    Dr. Chang Chuan-Fa

    Verified customer

    提交于 Feb 07 2012

    Question

    Sorry for disturbing. This customer indicated she already test this antibody and also submitted an abreview, therefore, she wants to know whether she can get one free primary antibody or not? Could you please help this customer to recheck this information?
    Thanks for your kindly help
    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Mar 05 2012

    Answer

    Thank you for contacting us back.

    Your Abreview has already been published, and the discount code then automatically activated. You can now place the order indicating the discount code on it.

    It is crucial to mention the discount code to get the free antibody.

    Thank you very much for sharing your results with us.

    Please do not hesitate to contact us for further assistance.

    Read More

    Abcam Scientific Support

    回复于 Mar 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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