生物素Anti-Myc tag抗体(ab34773)
Key features and details
- Biotin Rabbit polyclonal to Myc tag
- Suitable for: ELISA, WB
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-Myc tag抗体
参阅全部 Myc tag 一抗 -
描述
生物素兔多克隆抗体to Myc tag -
宿主
Rabbit -
偶联物
Biotin -
特异性
This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxytermini of targeted proteins in transfected or transformed cells. -
经测试应用
适用于: ELISA, WBmore details -
免疫原
Synthetic peptide corresponding to Myc tag aa 410-419 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:EQKLISEEDL
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常规说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34773于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
1/10000.
|
|
WB | (2) |
Use a concentration of 0.5 - 1 µg/ml.
|
说明 |
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ELISA
1/10000. |
WB
Use a concentration of 0.5 - 1 µg/ml. |
靶标
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相关性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
细胞定位
Nuclear -
别名
- c-myc tag antibody
- Myc Epitope Tag antibody
图片
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Mouse NIH 3T3 cells over-expressing pGEN IRES pyk2 Myc-tag. ab34773 was used at 1/2500 dilution. Please see accompanying abreview for additional information.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab34773 被引用在 2 文献中.
- Chakraborty M et al. Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity. Nat Commun 10:1673 (2019). PubMed: 30975984
- Hayes HL et al. Pdx-1 activates islet a- and ß-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2. Mol Cell Biol 33:4017-29 (2013). PubMed: 23938296