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Tags & Cell Markers Epitope Tags Conjugates

生物素Anti-Fluorescein抗体[BT.6A4] (ab6215)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

Product price, shipping and contact information

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Key features and details

  • Biotin Mouse monoclonal [BT.6A4] to Fluorescein
  • Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgM

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概述

  • 产品名称

    生物素Anti-Fluorescein抗体[BT.6A4]
    参阅全部 Fluorescein 一抗
  • 描述

    生物素小鼠单克隆抗体[BT.6A4] to Fluorescein
  • 宿主

    Mouse
  • 偶联物

    Biotin
  • 特异性

    The antibody reacts with free and bound fluorescein.
  • 经测试应用

    适用于: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details
  • 种属反应性

    与反应: Species independent
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯化说明

    Purified anti-fluorescein biotin conjugate.
  • 克隆

    单克隆
  • 克隆编号

    BT.6A4
  • 骨髓瘤

    unknown
  • 同种型

    IgM
  • 轻链类型

    kappa
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6215于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/5000.
ELISA
1/1000 - 1/10000.
IHC-P
1/200 - 1/2000.
IHC-Fr
1/200 - 1/2000.
In situ hybridization
Use at an assay dependent dilution.
说明
WB
1/500 - 1/5000.
ELISA
1/1000 - 1/10000.
IHC-P
1/200 - 1/2000.
IHC-Fr
1/200 - 1/2000.
In situ hybridization
Use at an assay dependent dilution.

靶标

  • 相关性

    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 别名

    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

实验方案

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab6215?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6215 被引用在 1 文献中.

  • Mukaiyama M  et al. Non-electrophilic TRPA1 agonists, menthol, carvacrol and clotrimazole, open epithelial tight junctions via TRPA1 activation. J Biochem N/A:N/A (2020). PubMed: 32428205

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

We typically use an anti-fluoroscein HRP antibody for southern blotting and it seems that the anti-fluoroscein biotinylated antibody would be the most comparable. My question is what detection reagents do you suggest to be used with the anti-fluoroscein biotinylated antibody that you sell??

Read More

Abcam community

Verified customer

Asked on Aug 08 2001

Answer

You could detect the biotinylated antibody directly or use a secondary to the fluorescein antibody.

Read More

Abcam Scientific Support

回复于 Aug 08 2001

Question

Has your anti fluorescein antibody be tested in Flow cytometry? I am thinking about labeling cells with CFSE which labels the cytoplasm. Over time the signal weakens and I was hoping to increase the signal by using an anti fluorescein mAb after I permiablize the cells.

Read More

Abcam community

Verified customer

Asked on Mar 09 2001

Answer

This antibody has not been tested in flow cytometry. However, in theory it should work in your application.

Read More

Abcam Scientific Support

回复于 Mar 10 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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