生物素Anti-Fibronectin抗体(ab6584)
Key features and details
- Biotin Rabbit polyclonal to Fibronectin
- Suitable for: IHC-P, WB
- Reacts with: Cow, Human, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
概述
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产品名称
生物素Anti-Fibronectin抗体
参阅全部 Fibronectin 一抗 -
描述
生物素兔多克隆抗体to Fibronectin -
宿主
Rabbit -
偶联物
Biotin -
特异性
negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Cow, Human, Recombinant fragment
预测可用于: Mammals
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免疫原
Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE
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常规说明
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. -
Primary antibody说明
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
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Related Products
应用
Our Abpromise guarantee covers the use of ab6584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/50 - 1/200. | |
| WB | 1/5000 - 1/10000. |
靶标
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功能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
组织特异性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
疾病相关
Glomerulopathy with fibronectin deposits 2 -
序列相似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
发展阶段
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻译后修饰
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 280794 Cow
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P07589 Cow
- SwissProt: P02751 Human
- Unigene: 203717 Human
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别名
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
图片
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Western blot - Biotin Anti-Fibronectin antibody (ab6584)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 6.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 9.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.
实验方案
文献 (38)
ab6584 被引用在 38 文献中.
- Moore MC et al. Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care. J Biomed Mater Res B Appl Biomater N/A:N/A (2020). PubMed: 32458591
- Gay D et al. Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing. Sci Adv 6:eaay3704 (2020). PubMed: 32219160
- Alcalde-Estévez E et al. Endothelin-1 induces cellular senescence and fibrosis in cultured myoblasts. A potential mechanism of aging-related sarcopenia. Aging (Albany NY) 12:11200-11223 (2020). PubMed: 32572011
- Carminucci A et al. Teaching an Old Drug New Tricks: Dexamethasone as an In Vivo Inhibitor of Glioblastoma Dispersal. Cureus 12:e7749 (2020). PubMed: 32455066
- Yemanyi F et al. Glucocorticoid-induced cell-derived matrix modulates transforming growth factor ß2 signaling in human trabecular meshwork cells. Sci Rep 10:15641 (2020). PubMed: 32973273
