生物素Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[FG4R] (ab173832)
Key features and details
- Biotin Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG2b
Related conjugates and formulations
概述
-
产品名称
生物素Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体[FG4R]
参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗 -
描述
生物素小鼠单克隆抗体[FG4R] to DDDDK tag (Binds to FLAG® tag sequence) -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
Sequence:DYKDDDDK
-
阳性对照
- E. coli lysate containing a multi-epitope tagged protein.
-
常规说明
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
FG4R -
同种型
IgG2b -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173832于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration.
|
说明 |
---|
WB
Use at an assay dependent concentration. |
靶标
-
相关性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
别名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
图片
-
All lanes : Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
Secondary
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.Note: Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Incubation with ab173832 was for 1 hour at room temperature. Incubation with secondary antibody was for 30 miutes at room temperature.
实验方案
数据表及文件
-
Datasheet download
文献 (0)
ab173832 尚未被引用在任何文献中。