生物素Anti-beta Galactosidase抗体(ab6645)

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Western blot - Biotin Anti-beta Galactosidase antibody (ab6645)

    Key features and details

    • Biotin Rabbit polyclonal to beta Galactosidase
    • Suitable for: Dot blot, ELISA, WB, IHC-P
    • Reacts with: Escherichia coli
    • Conjugation: Biotin
    • Isotype: IgG

    概述

    • 产品名称

      生物素Anti-beta Galactosidase抗体
      参阅全部 beta Galactosidase 一抗

    • 描述

      生物素兔多克隆抗体to beta Galactosidase

    • 宿主

      Rabbit

    • 偶联物

      Biotin

    • 经测试应用

      适用于: Dot blot, ELISA, WB, IHC-Pmore details

    • 种属反应性

      与反应: Escherichia coli

    • 免疫原

      Full length protein (E. coli).

    • 常规说明

      Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 纯度

      IgG fraction

    • 纯化说明

      This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab6645 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Dot blot Use at an assay dependent concentration.
    ELISA 1/10000 - 1/50000.
    WB 1/2000 - 1/10000.
    IHC-P 1/1000 - 1/5000.

    靶标

    • 相关性

      Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

    • 细胞定位

      Cytoplasmic

    • 数据库链接

      • 别名

        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      图片

      • Western blot - Biotin Anti-beta Galactosidase antibody (ab6645)
        Western blot - Biotin Anti-beta Galactosidase antibody (ab6645)
        Biotin Anti-beta Galactosidase antibody (ab6645) at 1/1000 dilution + Beta Galactosidase at 0.05 µg with Blocking buffer

        Secondary
        HRP streptavidin conjugate at 1/40000 dilution

      文献 (4)

      发表研究结果有使用 ab6645？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6645 被引用在 4 文献中.

      • Matsumoto K  et al. In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay). Sci Rep 7:12136 (2017). PubMed: 28939819
      • Herrera JJ  et al. Sustained expression of vascular endothelial growth factor and angiopoietin-1 improves blood-spinal cord barrier integrity and functional recovery after spinal cord injury. J Neurotrauma 27:2067-76 (2010). IHC-Fr ; Rat . PubMed: 20799882
      • Siembab VC  et al. Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells. J Comp Neurol 518:4675-701 (2010). PubMed: 20963823
      • Glaser T  et al. Generation of purified oligodendrocyte progenitors from embryonic stem cells. FASEB J 19:112-4 (2005). PubMed: 15486057

      客户评价及客户问答

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      1-4 of 4 Abreviews or Q&A

      Question

      I would like to use beta-gal antibody on the mouse tissue which has been processed in 10% formalin. Which beta-gal biotinylated antibody would be most suitable for the purchase. Thank you kindly

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      Answer

      I would recommend ab6645. We do not know if this antibody has been used in formalin treated tissue, however it has been used with IHC and I would expect it to work with formalin treated tissue as it is a polyclonal and thus recognises multiple epitopes.

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      Question

      Have you or anyone you know used this antibody for Flow cytometry? I was thinking about using it as a primary followed by your Donkey antirabbit-PE polyclonal? Do you have any staining protocols to stain blood cells?

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      Answer

      This antibody has not yet been tested in Flow Cytometry, therefore we can't guarantee that it will work. For a protocol regarding the staining of red blood cells, please see the general FACS protocol on the Abcam website

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      Question

      We have some formalin-fixed parafin embedded tissue that we would like to perform immunohistochemistry on using your Rabbit polyclonal to beta-galactosidase (Biotin) antibody -- ab6645. Since they are fixed with formalin initially, what would you recommend to obtain optimal antigen retrieval, i.e. products, protocol, etc.? Which secondary antibody would you highly recommend? Thank you.

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      Answer

      This antibody is biotinylated so there will be no need for a secondary antibody. You only require an ABC kit and chromogen. I recommend ab8621 for the ABC kit (Super ABC / Horseradish Peroxidase Basic Kit) and ab8184 (HRP Chromogen kit (DAB)) for the chromogen. I will personally e-mail you my antigen retrieval protocol. Try the pressure cooker method for starters.

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      Question

      Hello, I know this sounds like a silly question, but is this antibody biotinylated?

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      Answer

      Yes!

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