生物素Anti-beta Galactosidase抗体(ab6645)

Submit a review Q&A (4)References (4)
Western blot - Biotin Anti-beta Galactosidase antibody (ab6645)

    Key features and details

    • Biotin Rabbit polyclonal to beta Galactosidase
    • Suitable for: Dot blot, ELISA, WB, IHC-P
    • Reacts with: Escherichia coli
    • Conjugation: Biotin
    • Isotype: IgG

    概述

    • 产品名称

      生物素Anti-beta Galactosidase抗体
      参阅全部 beta Galactosidase 一抗

    • 描述

      生物素兔多克隆抗体to beta Galactosidase

    • 宿主

      Rabbit

    • 偶联物

      Biotin

    • 经测试应用

      适用于: Dot blot, ELISA, WB, IHC-Pmore details

    • 种属反应性

      与反应: Escherichia coli

    • 免疫原

      Full length protein (E. coli).

    • 常规说明

      Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
    • Concentration information loading...

    • 纯度

      IgG fraction

    • 纯化说明

      This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6645于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    WB
    1/2000 - 1/10000.
    IHC-P
    1/1000 - 1/5000.
    说明
    Dot blot
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    WB
    1/2000 - 1/10000.
    IHC-P
    1/1000 - 1/5000.

    靶标

    • 相关性

      Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.

    • 细胞定位

      Cytoplasmic

    • 数据库链接

      • 别名

        • Beta D galactosidase antibody
        • Beta gal antibody
        • Beta galactosidase antibody
        • ECK0341 antibody
        • JW0335 antibody
        • Lactase antibody
        • lacZ antibody
        see all

      图片

      • Western blot - Biotin Anti-beta Galactosidase antibody (ab6645)
        Western blot - Biotin Anti-beta Galactosidase antibody (ab6645)
        Biotin Anti-beta Galactosidase antibody (ab6645) at 1/1000 dilution + Beta Galactosidase at 0.05 µg with Blocking buffer

        Secondary
        HRP streptavidin conjugate at 1/40000 dilution

      文献 (4)

      发表研究结果有使用 ab6645？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6645 被引用在 4 文献中.

      • Matsumoto K  et al. In vivo target exploration of apidaecin based on Acquired Resistance induced by Gene Overexpression (ARGO assay). Sci Rep 7:12136 (2017). PubMed: 28939819
      • Herrera JJ  et al. Sustained expression of vascular endothelial growth factor and angiopoietin-1 improves blood-spinal cord barrier integrity and functional recovery after spinal cord injury. J Neurotrauma 27:2067-76 (2010). IHC-Fr ; Rat . PubMed: 20799882
      • Siembab VC  et al. Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells. J Comp Neurol 518:4675-701 (2010). PubMed: 20963823
      • Glaser T  et al. Generation of purified oligodendrocyte progenitors from embryonic stem cells. FASEB J 19:112-4 (2005). PubMed: 15486057

      客户评价及客户问答

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      1-4 of 4 Abreviews or Q&A

      Question

      I would like to use beta-gal antibody on the mouse tissue which has been processed in 10% formalin. Which beta-gal biotinylated antibody would be most suitable for the purchase. Thank you kindly

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      Answer

      I would recommend ab6645. We do not know if this antibody has been used in formalin treated tissue, however it has been used with IHC and I would expect it to work with formalin treated tissue as it is a polyclonal and thus recognises multiple epitopes.

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      Question

      Have you or anyone you know used this antibody for Flow cytometry? I was thinking about using it as a primary followed by your Donkey antirabbit-PE polyclonal? Do you have any staining protocols to stain blood cells?

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      Answer

      This antibody has not yet been tested in Flow Cytometry, therefore we can't guarantee that it will work. For a protocol regarding the staining of red blood cells, please see the general FACS protocol on the Abcam website

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      Question

      We have some formalin-fixed parafin embedded tissue that we would like to perform immunohistochemistry on using your Rabbit polyclonal to beta-galactosidase (Biotin) antibody -- ab6645. Since they are fixed with formalin initially, what would you recommend to obtain optimal antigen retrieval, i.e. products, protocol, etc.? Which secondary antibody would you highly recommend? Thank you.

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      Answer

      This antibody is biotinylated so there will be no need for a secondary antibody. You only require an ABC kit and chromogen. I recommend ab8621 for the ABC kit (Super ABC / Horseradish Peroxidase Basic Kit) and ab8184 (HRP Chromogen kit (DAB)) for the chromogen. I will personally e-mail you my antigen retrieval protocol. Try the pressure cooker method for starters.

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      Question

      Hello, I know this sounds like a silly question, but is this antibody biotinylated?

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      Answer

      Yes!

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