Key features and details
- Goat polyclonal to Biotin (Alkaline Phosphatase)
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
参阅全部 Biotin 一抗
经测试应用适用于: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details
种属反应性与反应: Species independent
Chemical/ Small Molecule conjugated to KLH
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.1% Sodium azide
Constituents: 0.87% Sodium chloride, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine), 1% BSA
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
Our Abpromise guarantee covers the use of ab6652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|WB||1/1000 - 1/3000.|
相关性Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
ab6652 被引用在 1 文献中.
- Paul A et al. Streptavidin-coated magnetic beads for DNA strand separation implicate a multitude of problems during cell-SELEX. Oligonucleotides 19:243-54 (2009). WB . PubMed: 19732022