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Anti-Biotin抗体(ab6643)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (7)

Product price, shipping and contact information

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Key features and details

  • Goat polyclonal to Biotin
  • Suitable for: ELISA, Immunodiffusion, IP
  • Reacts with: Species independent
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Biotin抗体
    参阅全部 Biotin 一抗
  • 描述

    山羊多克隆抗体to生物素
  • 宿主

    Goat
  • 经测试应用

    适用于: ELISA, Immunodiffusion, IPmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6643于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/20000 - 1/50000.
Immunodiffusion
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
说明
ELISA
1/20000 - 1/50000.
Immunodiffusion
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

靶标

  • 相关性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 别名

    • Vitamin B4 antibody

实验方案

  • Immunoprecipitation protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (7)

发表研究结果有使用 ab6643?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6643 被引用在 7 文献中.

  • Andresini O  et al. The long non-coding RNA Kcnq1ot1 controls maternal p57 expression in muscle cells by promoting H3K27me3 accumulation to an intragenic MyoD-binding region. Epigenetics Chromatin 12:8 (2019). PubMed: 30651140
  • Boucher D  et al. Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity. J Exp Med 215:827-840 (2018). PubMed: 29432122
  • Omeragic A  et al. Peroxisome proliferator-activated receptor-gamma: potential molecular therapeutic target for HIV-1-associated brain inflammation. J Neuroinflammation 14:183 (2017). PubMed: 28886715
  • Wu RA  et al. Single-molecule imaging of telomerase reverse transcriptase in human telomerase holoenzyme and minimal RNP complexes. Elife 4:N/A (2015). WB . PubMed: 26457608
  • Leduc F  et al. Genome-wide mapping of DNA strand breaks. PLoS One 6:e17353 (2011). IP . PubMed: 21364894
  • Best JM  et al. Small GTPase Rab11b regulates degradation of surface membrane L-type Cav1.2 channels. Am J Physiol Cell Physiol 300:C1023-33 (2011). WB . PubMed: 21248079
  • Mariner PD  et al. Human Alu RNA is a modular transacting repressor of mRNA transcription during heat shock. Mol Cell 29:499-509 (2008). PubMed: 18313387

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Question

Thank you very much for your answer. But, would you recomend me anyone in special, because I do not know which are the differences among them. I would appreciate a lot if you can tell me the differences among them. How many days do you need to make the antibody arriving me? Thank you again,

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Abcam community

Verified customer

Asked on Jul 17 2006

Answer

I have looked in detail at the datasheets for all three antibodies and the differences are: 1) the species in which they are raised in: -ab6643 and ab34579 are raised in goat -ab34552 and ab34826 are raised in rabbit 2) the way the antibodies were purified. -ab6643 was purified using biotin coupled to sepharose beads (high quality purification) -ab34552 was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer -ab34579 was purified from monospecific antiserum by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -ab34826 was purified from monoserum delipidified and defibrinated. This will affect the amount of antibody supplied per vial (so for the same price of €330 per vial we sell 0.1mg of ab6643 (the highly purified product) or 10 mg of ab34579 (less purified version). All antibodies except ab6643 are not in stock and will take 1-2 weeks to arrive following your order. Ab6643 is in stock and will take 1-2 days to arrive. I hope this information will help you decide which product is most suitable for your needs,

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Abcam Scientific Support

回复于 Jul 18 2006

Question

I need an anti-biotin antibody for immunoprecipitation with protein A or G magnetic microbeads (Miltenyi Biotec). Any of the following would work ok for it?: ab6643, ab34552, ab34579, ab34826.

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Abcam community

Verified customer

Asked on Jul 14 2006

Answer

Thank you for your enquiry. None of the antibodies ab6643, ab34552, ab34579 and ab34826 have been tested in IP with magnetic microbeads so I cannot guarantee they will work with those beads. HOwever I can confirm that they work with protein A and G sepharose beads. If in your experience these beads work in similar ways we can predict they may work in your beads. I'm sorry I couldn't help you more on this occasion,

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Abcam Scientific Support

回复于 Jul 17 2006

Question

How was this antibody tested in IHC-P? What was the dilution used, and for how long and at what temperature was it incubated?

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Abcam community

Verified customer

Asked on Feb 27 2006

Answer

Thank you for your enquiry. I apologize, but it appears that there was a typo on this datasheet. The antibody has not been tested in IHC. I am very sorry for the inconvenience. I have updated the datasheet to reflect the correct information. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

回复于 Feb 28 2006

Question

What is the pI of anti-biotin?

Read More

Abcam community

Verified customer

Asked on Jun 19 2002

Answer

This is an IgG antibody. We have never done any isoelectric point studies ourselves, but according to a website I have found, the isoelectric point of IgG is pH 6.1 - 8.5. I hope this is useful!

Read More

Abcam Scientific Support

回复于 Jun 20 2002

Question

Do you know if this antibody can be used as a marker for mitochondria in immunofluorescence assays?

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Abcam community

Verified customer

Asked on Dec 28 2001

Answer

I am unsure why you would wish to use this antibody (Goat polyclonal to Biotin) for this purpose. Do you have a biotin-labelled protein that localises specifically to the mitochondrion? You would have to use an anti-goat secondary conjugated to a fluorescent tag.

Read More

Abcam Scientific Support

回复于 Jan 03 2002

Question

what is the biotin binding capacity of this affinity purified Ab?

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Abcam community

Verified customer

Asked on Aug 13 2001

Answer

Sorry, we have no data on the biotin binding capacity.

Read More

Abcam Scientific Support

回复于 Aug 14 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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