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Anti-Biotin抗体[39-15D9] (ab3780)

Submit a review Q&A (4)References (2)

Key features and details

  • Mouse monoclonal [39-15D9] to Biotin
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-Biotin抗体[39-15D9]
    参阅全部 Biotin 一抗

  • 描述

    小鼠单克隆抗体[39-15D9] to生物素

  • 宿主

    Mouse

  • 特异性

    This antibody reacts with both free biotin and biotin bound to a carrier protein. There is no cross reaction with the free carrier protein (determined by ELISA).

  • 经测试应用

    适用于: ELISA, WBmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3780于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/5000 - 1/10000.
WB
1/1000 - 1/5000.
说明
ELISA
1/5000 - 1/10000.
WB
1/1000 - 1/5000.

靶标

  • 相关性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • 别名

    • Vitamin B4 antibody

文献 (2)

发表研究结果有使用 ab3780？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3780 被引用在 2 文献中.

  • Potluri LP  et al. ZipA is required for FtsZ-dependent preseptal peptidoglycan synthesis prior to invagination during cell division. J Bacteriol 194:5334-42 (2012). PubMed: 22843850
  • Ballard PL  et al. Regulated gene expression in cultured type II cells of adult human lung. Am J Physiol Lung Cell Mol Physiol 299:L36-50 (2010). PubMed: 20382749

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Question

Will this antibody bind the d-isomer of biotin?

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Answer

Thank you for your enquiry. This antibody does bind the d-isomer of biotin. Please do not hesitate to contact us if you have additional questions.

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Question

I am interested in your anti Biotin antibody (ab3780). Is there any Reference for this product? Where does the hybridoma cell line come from?

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Answer

Thank you for your enquiry. The hybridoma cell line comes from the antibody's originator (the source's name is proprietary) and unfortunately we do not have any references at this time. If you have any additional questions, please contact us again.

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Question

Having ordered your anti biotin antibody(ab3780) to use in a protein capture experiment,I find no information on the data sheet as to the concentration of the immunoglobulin in the ascites fluid.Is this just an omission? This makes quantitation of my experiments very difficult.any feedback?

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Answer

Thank you for your enquiry. We are sorry to inform you that there is no concentration of the immunoglobulin for antibodies that are not affinity purified. Please take a look at the suggested dilutions for each application listed on the datasheet.

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Question

what size should biotin be on a western blot

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Answer

On Western blot membran you can detect biotinylated proteins but not free biotine. Therefore the size of the bands depends of the molecular weight of the biotintylated protein.

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