Anti-Biotin抗体[39-15D9] (ab3780)

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Key features and details

  • Mouse monoclonal [39-15D9] to Biotin
  • Suitable for: ELISA, WB
  • Reacts with: Species independent
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-Biotin抗体[39-15D9]
    参阅全部 Biotin 一抗

  • 描述

    小鼠单克隆抗体[39-15D9] to生物素

  • 宿主

    Mouse

  • 特异性

    This antibody reacts with both free biotin and biotin bound to a carrier protein. There is no cross reaction with the free carrier protein (determined by ELISA).

  • 经测试应用

    适用于: ELISA, WBmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Full length protein conjugated to KLH.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab3780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000 - 1/10000.
WB 1/1000 - 1/5000.

靶标

  • 相关性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • 别名

    • Vitamin B4 antibody

文献 (2)

发表研究结果有使用 ab3780？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab3780 被引用在 2 文献中.

  • Potluri LP  et al. ZipA is required for FtsZ-dependent preseptal peptidoglycan synthesis prior to invagination during cell division. J Bacteriol 194:5334-42 (2012). PubMed: 22843850
  • Ballard PL  et al. Regulated gene expression in cultured type II cells of adult human lung. Am J Physiol Lung Cell Mol Physiol 299:L36-50 (2010). PubMed: 20382749

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Question

Will this antibody bind the d-isomer of biotin?

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Answer

Thank you for your enquiry. This antibody does bind the d-isomer of biotin. Please do not hesitate to contact us if you have additional questions.

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Question

I am interested in your anti Biotin antibody (ab3780). Is there any Reference for this product? Where does the hybridoma cell line come from?

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Answer

Thank you for your enquiry. The hybridoma cell line comes from the antibody's originator (the source's name is proprietary) and unfortunately we do not have any references at this time. If you have any additional questions, please contact us again.

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Question

Having ordered your anti biotin antibody(ab3780) to use in a protein capture experiment,I find no information on the data sheet as to the concentration of the immunoglobulin in the ascites fluid.Is this just an omission? This makes quantitation of my experiments very difficult.any feedback?

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Answer

Thank you for your enquiry. We are sorry to inform you that there is no concentration of the immunoglobulin for antibodies that are not affinity purified. Please take a look at the suggested dilutions for each application listed on the datasheet.

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Question

what size should biotin be on a western blot

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Answer

On Western blot membran you can detect biotinylated proteins but not free biotine. Therefore the size of the bands depends of the molecular weight of the biotintylated protein.

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