生物素Anti-6X His tag®抗体[HIS.H8] (ab173828)
Key features and details
- Biotin Mouse monoclonal [HIS.H8] to 6X His tag®
- Suitable for: ELISA, IP, ICC/IF, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
生物素Anti-6X His tag®抗体[HIS.H8]
参阅全部 6X His tag® 一抗 -
描述
生物素小鼠单克隆抗体[HIS.H8] to 6X His tag® -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: ELISA, IP, ICC/IF, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag® aa 1-6.
Sequence:HHHHHH
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阳性对照
- E. coli lysate containing a multi-epitope tagged protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
HIS.H8 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173828于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
1/500 - 1/2000.
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WB |
1/500 - 1/1000. Predicted molecular weight: 0.8 kDa.
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说明 |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
1/500 - 1/2000. |
WB
1/500 - 1/1000. Predicted molecular weight: 0.8 kDa. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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All lanes : Biotin Anti-6X His tag® antibody [HIS.H8] (ab173828) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.5 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 6 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
Secondary
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 0.8 kDaNote: Predicted MW is for the His tag alone.
实验方案
数据表及文件
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Datasheet download
文献 (0)
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