Anti-BIN1抗体(ab153912)
Key features and details
- Rabbit polyclonal to BIN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-BIN1抗体
参阅全部 BIN1 一抗 -
描述
兔多克隆抗体to BIN1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human BIN1 aa 1-300.
Database link: O00499-1 -
阳性对照
- A431 and Mouse kidney whole cell lysates; Human U87 xenograft tissue; A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab153912于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. -
组织特异性
Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle. -
疾病相关
Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. -
序列相似性
Contains 1 BAR domain.
Contains 1 SH3 domain. -
翻译后修饰
Phosphorylated by protein kinase C. -
细胞定位
Cytoplasm and Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 274 Human
- Entrez Gene: 30948 Mouse
- Omim: 601248 Human
- SwissProt: O00499 Human
- SwissProt: O08539 Mouse
- Unigene: 193163 Human
- Unigene: 4383 Mouse
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别名
- AMPH 2 antibody
- AMPH2 antibody
- Amphiphysin 2 antibody
see all
图片
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Anti-BIN1 antibody (ab153912) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 65 kDa
10% SDS PAGE -
Anti-BIN1 antibody (ab153912) at 1/500 dilution + Mouse kidney whole cell lysate at 50 µg
Predicted band size: 65 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling BIN1 with ab153912 at 1/500 dilution.
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Immunofluorescent analysis of A431 cells (fixed in iced cold MeOH; 5 mins) labeling BIN1 with ab153912 at 1/500 dilution (green). Right image shows cells co-stained with Hoechst 33342 (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab153912 被引用在 2 文献中.
- Crotti A et al. BIN1 favors the spreading of Tau via extracellular vesicles. Sci Rep 9:9477 (2019). PubMed: 31263146
- McAvoy KM et al. Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo. PLoS One 14:e0220125 (2019). PubMed: 31408457