Anti-Bid抗体(ab2388)

Submit a review Q&A (3)References (4)
Western blot - Anti-Bid antibody (ab2388)

    Key features and details

    • Rabbit polyclonal to Bid
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Bid antibody [Y8] (ab32060)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Bid抗体
      参阅全部 Bid 一抗

    • 描述

      兔多克隆抗体to Bid

    • 宿主

      Rabbit

    • 特异性

      This antibody detects full length (22 kDa) Bid.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide, surrounding amino acid 37 of Human Bid. (Peptide available as ab38270.)

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2388于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.
    说明
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.

    靶标

    • 功能

      The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

    • 组织特异性

      Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).

    • 结构域

      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

    • 翻译后修饰

      TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

    • 细胞定位

      Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Apoptic death agonist antibody
        • Apoptotic death agonist BID antibody
        • BH3 interacting domain death agonist antibody
        • BH3 interacting domain death agonist p11 antibody
        • BH3 interacting domain death agonist p13 antibody
        • BH3 interacting domain death agonist p15 antibody
        • BH3-interacting domain death agonist p11 antibody
        • BID antibody
        • BID isoform ES(1b) antibody
        • BID isoform L(2) antibody
        • BID isoform Si6 antibody
        • BID_HUMAN antibody
        • Desmocollin type 4 antibody
        • FP497 antibody
        • Human BID coding sequence antibody
        • MGC15319 antibody
        • MGC42355 antibody
        • p11 BID antibody
        • p13 BID antibody
        • p15 BID antibody
        • p22 BID antibody
        see all

      图片

      • Western blot - Anti-Bid antibody (ab2388)
        Western blot - Anti-Bid antibody (ab2388)
        Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours. Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours.

      文献 (4)

      发表研究结果有使用 ab2388？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2388 被引用在 4 文献中.

      • Subhawa S  et al. The Effects of Houttuynia cordata Thunb and Piper ribesioides Wall Extracts on Breast Carcinoma Cell Proliferation, Migration, Invasion and Apoptosis. Molecules 25:N/A (2020). PubMed: 32155880
      • Liu CL  et al. Reduced Nhe1 (Na+-H+ Exchanger-1) Function Protects ApoE-Deficient Mice From Ang II (Angiotensin II)-Induced Abdominal Aortic Aneurysms. Hypertension 76:87-100 (2020). PubMed: 32475310
      • Piao X  et al. 1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway. Front Pharmacol 9:372 (2018). PubMed: 29725297
      • Shinozaki-Ushiku A  et al. Profiling of Virus-Encoded MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma and Their Roles in Gastric Carcinogenesis. J Virol 89:5581-91 (2015). PubMed: 25740983

      客户评价及客户问答

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      Question

      I want to do immunostaining for cleaved BID (p15), whether this product can detect cleaved BID (p15)

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      Answer

      Thank you for your enquiry. Yes, this antibody detects both full length (22 kDa) and cleaved fragments (15 kDa) of Bid. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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      Question

      Can you send me an image? I am having a hard time with seeing multiple bands.

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      Answer

      Thank you for your enquiry. I have added an image of a Western blot using this antibody onto the datasheet. For your convenience, I have included a link to the datasheet at the bottom of this email. If you continue to have a problem with this antibody, please fill out the questionnaire located at the following link, and we will try to help you troubleshoot the problem: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=2388&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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      Question

      I want to know one of your primary antibody's immunogen. It will help me to know whether it is possible to react with chick. Its catalog number is ab2388. Thanks a lot!

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      Answer

      The immunogen is a synthetic peptide containing 15 amino acids around amino acids 40 of human Bid.

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