Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human BHLHB5.
(Peptide available as
This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse hippocampus; Mouse cerebellum; Mouse cortex; Mouse forebrain; Rat cerebellum.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).
Inhibits DNA binding of TCF3/E47 homodimers and TCF3 (E47)/NEUROD1 heterodimers and acts as a strong repressor of Neurod1 and Myod-responsive genes, probably by heterodimerization with class a basic helix-loop-helix factors. Despite the presence of an intact basic domain, does not bind to DNA.
Brain-specific, with the highest expression in the cerebellum.
Contains 1 basic helix-loop-helix (bHLH) domain.
Information by UniProt
Basic helix loop helix domain containing class B 5 1 2 5 antibody
Basic helix loop helix protein 5 antibody
Western blot - Anti-BHLHB5 antibody (ab95136)
All lanes :
Anti-BHLHB5 antibody (ab95136) at 1 µg/ml
Lane 1 :
Brain (Mouse) Tissue Lysate
Lane 2 :
Mouse Hippocampus Tissue Lysate
Lane 3 :
Cerebellum Mouse Tissue Lysate
Lane 4 :
mouse cortex Mouse Tissue Lysate
Lane 5 :
Forebrain (Mouse) Tissue Lysate
Lane 6 :
Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
38 kDa (
why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"