Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293T)
Specification
HEK 293T
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% saponin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1%BSA-PBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG Alexa568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000
Additional data
Additional Notes
Cells were grown on glass coverslips overnight and either stimulated with 100 mcM pervanadate or with PBS. Fixed with 2% PFA in PBS. Membrane permeablized with 0.5% saponin and beta-tubulin stained with anti-beta-tubulin and goat anti-rabbit Alexa 568. Coverslips were mounted to slides with ProLong Antifade and analyzed on a scanning laser confocal microscope (Leica).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Stefan Schattgen
Verified customer
提交于 Jan 15 2008