Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HepaRG, Huh7, HepG2)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
293T, HepaRG, Huh7, HepG2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBT 1x, Tween 0.1%, 5% Milk
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)–Peroxidase antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
/
Exposure
4 second(s)
Bands
Specific: 55 kDa
Positive control
293T, HepaRG, Huh7, HepG2
Additional data
Additional Notes
The best antibody for Tubulin detection.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Romain Parent
Verified customer
提交于 Oct 14 2020