Anti-beta Synuclein抗体(ab6165)

Reviews (1)Q&A (2)References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)

    Key features and details

    • Rabbit polyclonal to beta Synuclein
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-beta Synuclein抗体
      参阅全部 beta Synuclein 一抗

    • 描述

      兔多克隆抗体to beta Synuclein

    • 宿主

      Rabbit

    • 特异性

      Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species.

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide (IEPLMEPEGSYEDPPQE) corresponding to the carboxyl terminal of human beta-synuclein (108- 125) and was conjugated to diphtheria toxoid via a cystine residue added to the carboxyl terminus of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6165于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    1/500 - 1/2000.
    说明
    IHC-P
    1/500 - 1/2000.

    靶标

    • 功能

      Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.

    • 组织特异性

      Expressed predominantly in brain; concentrated in presynaptic nerve terminals.

    • 序列相似性

      Belongs to the synuclein family.

    • 翻译后修饰

      Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.

    • 细胞定位

      Cytoplasm.
    • Information by UniProt

    • 数据库链接

      • 别名

        • 14 kDa brain-specific protein antibody
        • Beta-synuclein antibody
        • Phosphoneuroprotein 14 antibody
        • PNP14 antibody
        • Sncb antibody
        • Synuclein beta antibody
        • SYUB_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
        ab6165 staining beta synuclein in human occipital cortex.
        Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
        Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      文献 (4)

      发表研究结果有使用 ab6165？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6165 被引用在 4 文献中.

      • Lahut S  et al. Blood RNA biomarkers in prodromal PARK4 and rapid eye movement sleep behavior disorder show role of complexin 1 loss for risk of Parkinson's disease. Dis Model Mech 10:619-631 (2017). PubMed: 28108469
      • Helwig M  et al. Brain propagation of transduced a-synuclein involves non-fibrillar protein species and is enhanced in a-synuclein null mice. Brain 139:856-70 (2016). WB ; Mouse . PubMed: 26719384
      • Beyer K  et al. The decrease of {beta}-synuclein in cortical brain areas defines a molecular subgroup of dementia with Lewy bodies. Brain : (2010). WB ; Human . PubMed: 20959308
      • Iwata A  et al. alpha-Synuclein affects the MAPK pathway and accelerates cell death. J Biol Chem 276:45320-9 (2001). WB ; Human . PubMed: 11560921

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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Synuclein antibody

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Brain, Cortex)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric Acid
      Permeabilization
      No
      Specification
      Brain, Cortex
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
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      Abcam user community

      Verified customer

      提交于 May 23 2018

      Question

      Can you please advise me on which blocking peptide to use against rabbit anti-beta synuclein (ab6165). We are using it in human tissue for immunohistochemistry.

      Please let me know if you require any further information.

      Looking forward for your response. When replying back can you please address the email “Dear Juli” as all replies come through a centralised email. This will avoid any confusion our end.

      Thanks

      Kind regards,

      Read More
      Answer

      Thank you for your enquiry regarding ab6165.

      I regret to inform you that the blocking peptide is not available commercially.

      Apologies!

      Read More

      Question

      Please see customers request below:

      Can you please advise me on which blocking peptide to use against rabbit anti-beta synuclein (ab6165). We are using it in human tissue for immunohistochemistry.

      Please let me know if you require any further information.

      Thanks

      Kind regards,

      Read More
      Answer

      Thank you for your email.

      I have contacted our laboratory and inquired about this.

      I will get back to you with the updates soon.

      Read More

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