Anti-Beta Lactamase抗体[8A5.A10] (ab12251)
Key features and details
- Mouse monoclonal [8A5.A10] to Beta Lactamase
- Suitable for: ELISA, WB
- Reacts with: Escherichia coli
- Isotype: IgG1
概述
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产品名称
Anti-Beta Lactamase抗体[8A5.A10] -
描述
小鼠单克隆抗体[8A5.A10] to Beta Lactamase -
宿主
Mouse -
特异性
This antibody specifically recognizes TEM type beta lactamases. -
经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Recombinant full length protein corresponding to Mouse Beta Lactamase.
Database link: P62593 -
常规说明
Dilute in PBS or medium which is identical to that used in the assay system.
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
8A5.A10 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12251于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use a concentration of 10 - 20 µg/ml.
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WB |
Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa.
(31.5kDa is the molecular weight of the unprocessed precursor.) |
说明 |
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ELISA
Use a concentration of 10 - 20 µg/ml. |
WB
Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa. (31.5kDa is the molecular weight of the unprocessed precursor.) |
靶标
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相关性
The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant. -
别名
- AmpA antibody
- AmpC antibody
- Cephalosporinase antibody
数据表及文件
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Datasheet download
文献 (27)
ab12251 被引用在 27 文献中.
- Chaoprasid P et al. Crystal structure of bacterial cytotoxic necrotizing factor CNFY reveals molecular building blocks for intoxication. EMBO J 40:e105202 (2021). PubMed: 33410511
- Naha A et al. Deciphering the possible role of ctxB7 allele on higher production of cholera toxin by Haitian variant Vibrio cholerae O1. PLoS Negl Trop Dis 14:e0008128 (2020). PubMed: 32236098
- Rivas ZP et al. CexE Is a Coat Protein and Virulence Factor of Diarrheagenic Pathogens. Front Microbiol 11:1374 (2020). PubMed: 32714302
- Xiong D et al. Salmonella Coiled-Coil- and TIR-Containing TcpS Evades the Innate Immune System and Subdues Inflammation. Cell Rep 28:804-818.e7 (2019). PubMed: 31315056
- Singh R et al. A ß-lactamase-producing plasmid from Neisseria gonorrhoeae carrying a unique 6 bp deletion in blaTEM-1 encoding a truncated 24 kDa TEM-1 penicillinase that hydrolyses ampicillin slowly. J Antimicrob Chemother 74:2904-2912 (2019). PubMed: 31335939