Application
Western blot
Sample
Rat Tissue lysate - whole (Brain lysate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Brain lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/10000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-tubulin
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Fluorescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50, 48 kDa
Positive control
Rat brain
Additional data
Additional Notes
beta-III-tubulin: 50kDa
tyrosine hydroxylase: 48kDa
Lane 1 + 3: Rat striatum lysate, samples.
Lane 2: Rat brain lysate, control.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 27 2018