Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (e6: developing eye.)
Specification
e6: developing eye.
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/1250
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Ab78078 (1/1250) staining Neuron specific beta III Tubulin (2G10) in Immunohistochemistry Paraffin-embedded Chicken e6 (developing eye) tissue section.
Excellent neurone-specific localisation with this antibody.
Image shows optic nerve entering/leaving the eye.
Excellent neurone-specific localisation with this antibody.
Image shows optic nerve entering/leaving the eye.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Mar 11 2010