Anti-beta Galactosidase抗体[DC1 4C7] (ab116)

Submit an Abreview Q&A (13)Specific References (5)

概述

  • 产品名称
    Anti-beta Galactosidase抗体[DC1 4C7]
    参阅全部 beta Galactosidase 一抗
  • 描述
    小鼠单克隆抗体[DC1 4C7] to beta Galactosidase
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ELISAmore details
  • 免疫原

    beta Galactosidase EC.3.2.1.23 from E.coli.

  • 常规说明

    This monoclonal is also known as clone HD4C7 or HD 447C.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    单克隆
  • 克隆编号
    DC1 4C7
  • 骨髓瘤
    P3-NS1/1-Ag4-1
  • 同种型
    IgG1
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

靶标

  • 相关性
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 细胞定位
    Cytoplasmic
  • 别名
    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all

图片

  • Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (ab116)
    Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (ab116)
    All lanes : Anti-beta Galactosidase antibody [DC1 4C7] (ab116) at 1 µg

    Lane 1 : Recombinant Human GLB1 protein (ab151890)
    Lane 2 : beta Galactosidase protein (ab79449)

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Performed under reducing conditions.

    Exposure time: 4 minutes

实验方案

文献

This product has been referenced in:
  • Smith SJ  et al. Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition. PLoS One 7:e52335 (2012). IHC-P . Read more (PubMed: 23272238) »
  • Nassiri M  et al. Repressors NFI and NFY participate in organ-specific regulation of von Willebrand factor promoter activity in transgenic mice. Arterioscler Thromb Vasc Biol 30:1423-9 (2010). IHC-Fr . Read more (PubMed: 20431063) »

See all 5 Publications for this product

发表研究结果有使用 ab116？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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13 reviews or Q&A

10
Votes: Highest First

We have bought mouse monoclonal [DC1 4c7] to beta Galactosidase (ab116) from your company. I trasnfected mammalian cells with Positive ctrl plasmid from Invitrogen(pcDNA4.0/Hismax/LacZ) which will generate a LacZ and His-tag fusion protein. Subsequentl...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We do not have a recommended dilution of this antibody. The dilution employed will depend on the level of expression of your LacZ construct. H...

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BATCH NUMBER 286 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal in all samples SAMPLE 1. ?-gal fusion protein from engineered ES cell 2. Over-expressed ?-gal 3. Purified ?-gal protein PR...

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Thank you for your enquiry and for submitting your technical questionnaire. I am sorry to hear that your customer has been having difficulties with this antibody. I have read their technical enquiry and they have tried some of the recommendations th...

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BATCH NUMBER 286 ORDER NUMBER ? DESCRIPTION OF THE PROBLEM No staining SAMPLE Mouse embryonic brain PRIMARY ANTIBODY Ab116 1:50, 1:100 and 1:400 SECONDARY ANTIBODY Dako goat anti-mouse 1:200 (works very well for other mou...

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Thank you for your enquiry and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are having problem with this antibody. We would like to confirm that this antibody has not been tested for immunohistochemi...

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Thank you very much for your reply. I just aware that your anti-b-gal is from rabbit. However, I am searching an anti-b-Gal with non-rabbit source, because I need it for double labeling with neural progenitor markers such as Sox-2, Sox-10 and Mash-1, w...

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Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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Thanks for your reply. To my understanding, the ab616 will be good for detecting b-GAL by immunohistochemistry at mouse brain. I shall appreciate it very much if you can provide further information. Best regards,

Yes, ab616 has successfully been tested for application in IHC-formalin fixed paraffin embedded sections. We have had some feedback from customers who have used ab616 in IHC-P to detect beta gal in transgenic mice - you may read their comments by click...

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I am a customer of your company. Could I ask you some questions concerning the antibodies ab116 and ab1047? Do you have any data to show which can be used for immunohistochemistry in tissue. I want to buy one of them for parafomaldehyde perfused mouse ...

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Thank you for your email. Neither ab116 or ab1047 have been tested yet for application in IHC. You may want to look at ab616 which has been tested in IHC. If you decide to try ab116 or ab1047 in IHC, please let us know how you get on and in return w...

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I would like to use an anti-beta-galactosidase antibody to detect a truncated beta-gal protein containing only the N terminus (the first 300 AA). Do you know if the epitope of your ab116 antibody is within this region. Thanks in advance for your time,

The epitope of this antibody has not been mapped.

Information suitability for immunocytochemistry.

This anitbody has not yet been tested in immunocytochemistry

Does the antibody detect ß Gal expression by eucaryotic cells in WB? Thank you.

Yes this antibody does work in this application (although there maybe some post-translational modification of the b-gal fusion protein which may decrease the binding of the antibody)

I would like to use an anti-beta-galactosidase antibody to detect E.coli beta-gal in an ELISA. Previously I have used Gibco clone 5B88 to coat the ELISA plates at a conc. of 2.5ug/ml. Can ab116 be used in such an application?

Yes, this antibody works in ELISA.

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