Anti-beta Galactosidase抗体(ab616)
Key features and details
- Rabbit polyclonal to beta Galactosidase
- Suitable for: ELISA, WB, IP
- Reacts with: Escherichia coli
- Isotype: IgG
概述
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产品名称
Anti-beta Galactosidase抗体
参阅全部 beta Galactosidase 一抗 -
描述
兔多克隆抗体to beta Galactosidase -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, IPmore details
不适用于: ICC/IF,IHC-Fr or IHC-P -
种属反应性
与反应: Escherichia coli -
免疫原
Recombinant full length protein (E. coli).
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常规说明
Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯度
IgG fraction -
纯化说明
IgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation, ion exchange chromatography and dialysis. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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ELISA |
1/10000.
sELISA tested against 1ug E.coli beta galactosidase. |
|
WB | (8) |
1/5000 - 1/10000. Detects a band of approximately 116 kDa.
This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives. |
IP |
1/500 - 1/5000.
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说明 |
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ELISA
1/10000. sELISA tested against 1ug E.coli beta galactosidase. |
WB
1/5000 - 1/10000. Detects a band of approximately 116 kDa. This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives. |
IP
1/500 - 1/5000. |
靶标
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相关性
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
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别名
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
图片
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All lanes : Anti-beta Galactosidase antibody (ab616)
Lanes 1-2 : Non-reduced purified recombinant beta Galactosidase protein
Lanes 3-4 : reduced purified recombinant beta Galactosidase proteinWestern blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (109)
ab616 被引用在 109 文献中.
- Péladeau C et al. Identification of therapeutics that target eEF1A2 and upregulate utrophin A translation in dystrophic muscles. Nat Commun 11:1990 (2020). PubMed: 32332749
- Qiao W et al. 1,25-Dihydroxyvitamin D insufficiency accelerates age-related bone loss by increasing oxidative stress and cell senescence. Am J Transl Res 12:507-518 (2020). PubMed: 32194899
- Hilbold E et al. Loss of Cx43 in Murine Sertoli Cells Leads to Altered Prepubertal Sertoli Cell Maturation and Impairment of the Mitosis-Meiosis Switch. Cells 9:N/A (2020). PubMed: 32164318
- de Vreede G et al. A Drosophila Tumor Suppressor Gene Prevents Tonic TNF Signaling through Receptor N-Glycosylation. Dev Cell 45:595-605.e4 (2018). PubMed: 29870719
- Park H et al. Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms. Nat Commun 9:5293 (2018). PubMed: 30546048