- 数据表
- 具体的参考文献 (94)
- 实验方案
概述
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产品名称Anti-beta Galactosidase抗体
参阅全部 beta Galactosidase 一抗 -
描述兔多克隆抗体to beta Galactosidase
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宿主Rabbit
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经测试应用适用于: ELISA, WB, IPmore details
不适用于: ICC/IF,IHC-FoFr,IHC-Fr or IHC-P -
种属反应性与反应: Escherichia coli
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免疫原
Recombinant full length protein (E. coli).
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阳性对照
- 1.0µg of E.coli in a standard sandwich ELISA. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified E.coli beta-galactosidase.
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常规说明
Application Note: This antibody shows inconsistent results between batches in ICC/IF, IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS.We would recommend ab4761 or ab12081 as alternatives.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.01% Sodium Azide
Constituents: 1% BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 -
Concentration information loading... -
纯度IgG fraction
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纯化说明IgG fraction purified from monospecific antiserum by a multi-step process of delipidation, salt fractionation, ion exchange chromatography and dialysis.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
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| ELISA | 1/10000. sELISA tested against 1ug E.coli beta galactosidase. |
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| WB | 1/5000 - 1/10000. Detects a band of approximately 116 kDa. This antibody shows inconsistent results between batches in ICC/IF, IHC-P, IHC-Fr and IHC-FoFr. We have received several good Abreviews on these applications, however, we are unable to guarantee positive results. If the antibody is used for IHC, please try fixing with methanol and block with gelatine instead with BSA or NGS. After each antibody incubation, please wash 5x with TBS instead of PBS. We would recommend ab4761 or ab12081 as alternatives. |
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| IP | 1/500 - 1/5000. |
靶标
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相关性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
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细胞定位Cytoplasmic
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数据库链接
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
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别名
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
图片
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Western blot - Anti-beta Galactosidase antibody (ab616)Western blotting using ab616. Lane 1 shows 80 ng and lane 2 shows 20 ng loaded onto gel. Results for non-reducing conditions of SDS-PAGE prior to transfer to nitrocellulose are shown on the left side of the figure; results obtainined under reducing conditions are shown on the right. The membrane was probed with ab616 at 1/10,000 dilution.
文献
This product has been referenced in:
- Chen ZJ et al. Renoprotective Effect of a Chinese Herbal Formula, Qidan Dihuang Decoction, on Streptozotocin-Induced Diabetes in Rat. Evid Based Complement Alternat Med 2018:7321086 (2018). Read more (PubMed: 29849726) »
- Chodelkova O et al. Tcf7L2 is essential for neurogenesis in the developing mouse neocortex. Neural Dev 13:8 (2018). IHC-P . Read more (PubMed: 29751817) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"