Anti-beta COP抗体(ab2899)
Key features and details
- Rabbit polyclonal to beta COP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Hamster, Cow, Human, Non human primates
- Isotype: IgG
概述
-
产品名称
Anti-beta COP抗体
参阅全部 beta COP 一抗 -
描述
兔多克隆抗体to beta COP -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Hamster, Cow, Human, Non human primates
预测可用于: Chicken, Pig
-
免疫原
Synthetic peptide corresponding to Rat beta COP aa 496-513.
Sequence:EAGELKPEEEITVGPVQK
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Ammonium Sulphate Precipitation -
Primary antibody说明
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
Our Abpromise guarantee covers the use of ab2899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 110 kDa. | |
| ICC/IF | 1/2000. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion. |
靶标
-
功能
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis (By similarity). Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments. -
序列相似性
Contains 6 HEAT repeats. -
翻译后修饰
Proteolytically cleaved between Ser-528 and Ser-529 by CAPN8. -
细胞定位
Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. Cell membrane. Endoplasmic reticulum-Golgi intermediate compartment. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Proteolytic cleavage by CAPN8 triggers translocation from Golgi to cytoplasm (By similarity). Found in perinuclear vesicular-tubular clusters (VTCs) and in the Golgi region where associated with vesicles, buds and rims of the Golgi stack (By similarity). Occasionally present at the trans-side of Golgi, but mainly present at the cis-Golgi side in transitional areas (TA), on so-called peripheral elements (PE) consisting of tubules and vesicles located between the cup-shaped transitional elements (TE) of the rough endoplasmic reticulum (RER) and the cis-most Golgi cisternae (By similarity). Present in cytoplasm, not associated with visible coats or membranes, with a minor fraction present on small clusters of tubules and vesicles (By similarity). Some association with high-density and low-density microsomes and mitochondria/nuclei fraction (By similarity). Very little found in plasma membrane fraction. - Information by UniProt
-
数据库链接
- Entrez Gene: 423063 Chicken
- Entrez Gene: 535533 Cow
- Entrez Gene: 1315 Human
- Entrez Gene: 70349 Mouse
- Entrez Gene: 100337660 Pig
- Entrez Gene: 114023 Rat
- Omim: 600959 Human
- SwissProt: Q5ZIA5 Chicken
see all -
别名
- Beta-coat protein antibody
- Beta-COP antibody
- betacop antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)Immunolocalization of beta COP in NIH-3T3 cells using ab2899
-
Western blot - Anti-beta COP antibody (ab2899)Western blot of beta-COP on rat brain homogenate using ab2899. Western blot of beta-COP on rat brain homogenate using ab2899.
实验方案
文献 (28)
ab2899 被引用在 28 文献中.
- Sasako T et al. Hepatic Sdf2l1 controls feeding-induced ER stress and regulates metabolism. Nat Commun 10:947 (2019). PubMed: 30814508
- Nair S et al. Adult stem cell deficits drive Slc29a3 disorders in mice. Nat Commun 10:2943 (2019). PubMed: 31270333
- Pocognoni CA et al. Highly conserved motifs within the large Sec7 ARF guanine nucleotide exchange factor GBF1 target it to the Golgi and are critical for GBF1 activity. Am J Physiol Cell Physiol 314:C675-C689 (2018). PubMed: 29443553
- Chen J et al. Impairment of Cargo Transportation Caused by gbf1 Mutation Disrupts Vascular Integrity and Causes Hemorrhage in Zebrafish Embryos. J Biol Chem 292:2315-2327 (2017). PubMed: 28003365
- Rafiq NB et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol 216:181-197 (2017). WB, IF . PubMed: 28007915
