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Signal Transduction Protein Trafficking Chaperones

Anti-beta COP抗体(ab2899)

  • Datasheet
  • SDS
Reviews (15)Q&A (1)References (28)

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Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)
  • Western blot - Anti-beta COP antibody (ab2899)

Key features and details

  • Rabbit polyclonal to beta COP
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Hamster, Cow, Human, Non human primates
  • Isotype: IgG

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概述

  • 产品名称

    Anti-beta COP抗体
    参阅全部 beta COP 一抗
  • 描述

    兔多克隆抗体to beta COP
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Hamster, Cow, Human, Non human primates
    预测可用于: Chicken, Pig
  • 免疫原

    Synthetic peptide corresponding to Rat beta COP aa 496-513.
    Sequence:

    EAGELKPEEEITVGPVQK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Ammonium Sulphate Precipitation
  • Primary antibody说明

    Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • BALB/3T3 whole cell lysate (ab7901)

应用

Our Abpromise guarantee covers the use of ab2899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 110 kDa.
ICC/IF 1/2000. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.

靶标

  • 功能

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis (By similarity). Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.
  • 序列相似性

    Contains 6 HEAT repeats.
  • 翻译后修饰

    Proteolytically cleaved between Ser-528 and Ser-529 by CAPN8.
  • 细胞定位

    Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. Cell membrane. Endoplasmic reticulum-Golgi intermediate compartment. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Proteolytic cleavage by CAPN8 triggers translocation from Golgi to cytoplasm (By similarity). Found in perinuclear vesicular-tubular clusters (VTCs) and in the Golgi region where associated with vesicles, buds and rims of the Golgi stack (By similarity). Occasionally present at the trans-side of Golgi, but mainly present at the cis-Golgi side in transitional areas (TA), on so-called peripheral elements (PE) consisting of tubules and vesicles located between the cup-shaped transitional elements (TE) of the rough endoplasmic reticulum (RER) and the cis-most Golgi cisternae (By similarity). Present in cytoplasm, not associated with visible coats or membranes, with a minor fraction present on small clusters of tubules and vesicles (By similarity). Some association with high-density and low-density microsomes and mitochondria/nuclei fraction (By similarity). Very little found in plasma membrane fraction.
  • Target information above from: UniProt accession P53618 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 423063 Chicken
    • Entrez Gene: 535533 Cow
    • Entrez Gene: 1315 Human
    • Entrez Gene: 70349 Mouse
    • Entrez Gene: 100337660 Pig
    • Entrez Gene: 114023 Rat
    • Omim: 600959 Human
    • SwissProt: Q5ZIA5 Chicken
    • SwissProt: A0JN39 Cow
    • SwissProt: P53618 Human
    • SwissProt: Q9JIF7 Mouse
    • SwissProt: D2SW95 Pig
    • SwissProt: P23514 Rat
    • Unigene: 339278 Human
    • Unigene: 277024 Mouse
    • Unigene: 4327 Rat
    see all
  • 别名

    • Beta-coat protein antibody
    • Beta-COP antibody
    • betacop antibody
    • Coatomer beta subunit antibody
    • Coatomer protein complex subunit beta 1 antibody
    • Coatomer protein complex subunit beta antibody
    • Coatomer subunit beta antibody
    • COPB antibody
    • COPB_HUMAN antibody
    • Copb1 antibody
    • DKFZp761K102 antibody
    • FLJ10341 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)
    Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)

    Immunolocalization of beta COP in NIH-3T3 cells using ab2899

  • Western blot - Anti-beta COP antibody (ab2899)
    Western blot - Anti-beta COP antibody (ab2899)
    Western blot of beta-COP on rat brain homogenate using ab2899. Western blot of beta-COP on rat brain homogenate using ab2899.

实验方案

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (28)

    发表研究结果有使用 ab2899?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2899 被引用在 28 文献中.

    • Sasako T  et al. Hepatic Sdf2l1 controls feeding-induced ER stress and regulates metabolism. Nat Commun 10:947 (2019). PubMed: 30814508
    • Nair S  et al. Adult stem cell deficits drive Slc29a3 disorders in mice. Nat Commun 10:2943 (2019). PubMed: 31270333
    • Pocognoni CA  et al. Highly conserved motifs within the large Sec7 ARF guanine nucleotide exchange factor GBF1 target it to the Golgi and are critical for GBF1 activity. Am J Physiol Cell Physiol 314:C675-C689 (2018). PubMed: 29443553
    • Chen J  et al. Impairment of Cargo Transportation Caused by gbf1 Mutation Disrupts Vascular Integrity and Causes Hemorrhage in Zebrafish Embryos. J Biol Chem 292:2315-2327 (2017). PubMed: 28003365
    • Rafiq NB  et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol 216:181-197 (2017). WB, IF . PubMed: 28007915
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 16 Abreviews or Q&A

    Western blot abreview for Anti-beta COP antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More

    Dr. Jerome Cattin

    Verified customer

    提交于 May 20 2020

    Western blot abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (retina)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    20 µg
    Specification
    retina
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 12 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice Hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice Hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Apr 02 2019

    Western blot abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mice Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    35 µg
    Specification
    Mice Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Feb 19 2019

    Western blot abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Podocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    Mouse Podocytes
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 27 2018

    Western blot abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (DU145 - prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    26 µg
    Specification
    DU145 - prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Sep 28 2017

    Western blot abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (4-15% SDS PAGE)
    Loading amount
    15 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Apr 07 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (RWPE - prostate cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    RWPE - prostate cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Mar 31 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    提交于 Mar 28 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta COP antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Formaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 02 2012

    1-10 of 16 Abreviews or Q&A

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