Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cell line)
Loading amount
30 µg
Specification
U2OS cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/5000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk 0.1% Tween-20 in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti-rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~90 kDa Non-specific: none kDa
Additional data
Additional Notes
High quality antibody
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 09 2007