概述

  • 产品名称
    Anti-beta Catenin抗体[E247]
    参阅全部 beta Catenin 一抗
  • 描述
    兔单克隆抗体[E247] to beta Catenin
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-Fr, IHC-P, WB, ICC/IF, IP, ChIPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Hamster, Cow, Human, Macaque monkey, African green monkey
  • 免疫原

    Synthetic peptide within Human beta Catenin aa 1-100 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: A431, HeLa and wild-type HAP1 cell lysate. ICC/IF: A431 and wild-type HAP1 cells. SW480 and SK-N-SH cells. IHC-P: Human lung adenocarcinoma, kidney adenocarcinoma, colon adenocarcinoma, cervical carcinoma, breast carcinoma and papillary carcinoma of thyroid gland tissue.
  • 常规说明

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/200.
IHC-P 1/500.
WB 1/5000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 86 kDa).

We recommend Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773).

ICC/IF 1/250.

We recommend Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody.

IP 1/100.
ChIP Use at an assay dependent concentration. PubMed: 28923827
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 功能
      Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
      Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
    • 组织特异性
      Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
    • 疾病相关
      Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
      Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
      Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
      Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
      Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
      Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
    • 序列相似性
      Belongs to the beta-catenin family.
      Contains 12 ARM repeats.
    • 翻译后修饰
      Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
      EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
      Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
    • 细胞定位
      Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Beta catenin antibody
      • Beta-catenin antibody
      • Cadherin associated protein antibody
      • Catenin (cadherin associated protein), beta 1, 88kDa antibody
      • Catenin beta 1 antibody
      • Catenin beta-1 antibody
      • CATNB antibody
      • CHBCAT antibody
      • CTNB1_HUMAN antibody
      • CTNNB antibody
      • CTNNB1 antibody
      • DKFZp686D02253 antibody
      • FLJ25606 antibody
      • FLJ37923 antibody
      • OTTHUMP00000162082 antibody
      • OTTHUMP00000165222 antibody
      • OTTHUMP00000165223 antibody
      • OTTHUMP00000209288 antibody
      • OTTHUMP00000209289 antibody
      see all

    图片

    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: CTNNB1 (β-catenin) knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: A431 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32572 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32572 was shown to specifically react with CTNNB1 (β-catenin) in wild type HAP1 cells. No band was observed when CTNNB1 (β-catenin) knockout samples were used. Wild-type and CTNNB1 (β-catenin) knockout samples were subjected to SDS-PAGE. ab32572 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/5000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • ab32572 staining in CTNNB1 (beta Catenin) wild-type HAP1 cells (top panel) and in CTNNB1 (β-catenin) knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab32572 at 1/250 dilution and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 2 μg/ml (shown in green). Nuclear DNA was labeled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Different expression level of beta Catenin in HCTs (hepatocellular carcinoma tissues) and PLTs (para-cancerous liver tissues).

      The HCTs, PLTs were paraffin-embedded and cut into sections with 5 μm-thickness for hematoxylin-eosin and immunohistochemistry (IHC) analysis. ab32572 was used at a dilution of 1:400. The second antibody was a biotinylated IgG to incubate 40 minutes at 37°C. Finally, the tissue slices were visualized by the 3, 3-diaminobenzidine solution and counterstained with hematoxylin. Substitution of the primary antibody with phosphate-buffered saline was served as a control for IHC.

      The beta Catenin with negative, weak, moderate and strong staining activity was respectively detected in HCTs (E-H) and PLTs (M-P). Section E shown above, for full image please see original paper.

    • WB analysis of total cell extracts from WT and gene disrupted cells using ab32572 at a 1/5000 dilution together with anti actin antibody. The position and full length β-catenin, truncated β-catenin and actin bands are indicated. For wild type cells 5 µg of TP and for the gene disrupted clones 30 µg of TP was applied for each lane.

      Cells were lysed in RIPA buffer containing protease inhibitor and phosphatase inhibitor tablets. Cell lysates were cleared by centrifugation and protein concentration 5–30 µg of total protein in SDS sample buffer was loaded per lane and separated.

      Secondary antibody was a donkey anti-rabbit IgG-HRP used at a 1:5000 dilution.

    • ab32572 staining beta Catenin in SW480 (Human colorectal adenocarcinoma cell line) cells treated with BIO (ab120891), by ICC/IF. Increase of beta Catenin expression correlates with increased concentration of BIO, as described in literature.
      The cells were incubated at 37°C for 48h in media containing different concentrations of ab120891 (BIO) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32572 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899) secondary antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    • ab32572 showing positive staining in human cervical carcinoma tissue.

    • ab32572 showing positive staining in human breast carcinoma tissue.

    • ab32572 showing positive staining in human papillary carcinoma of thyroid gland tissue.

    • ab32572 showing positive staining in human lung adenocarcinoma tissue.

    • ab32572 showing positive staining in human kidney carcinoma tissue.

    • ab32572 staining beta Catenin in SK-N-SH (Human neuroblastoma cell line) cells treated with olanzapine (ab120736), by ICC/IF.

      Increase in expression of beta Catenin correlates with increased concentration of olanzapine, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120736 (olanzapine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32572 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899) secondary antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    • ab32572 staining beta Catenin in the bEnd.5 murine cell line by ICC/IF (Immunocytochemistry/immunofluorescence).

      Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton in PBS and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/300) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • ab32572 staining beta Catenin in dog colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10 mM citrate buffer, pH6. Samples were incubated with primary antibody (1/250 in PBS with 1x casein) for 90 minutes at 25°C. A biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Western blot image of ab32572 staining whole cell lysate of U-2 OS (Human bone osteosarcoma epithelial cell line) cells.  The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted 1/5000 and incubated  for 12 hours at 4°C.  An HRP conjugated swine anti-rabbit antibody was used as the secondary.

      See Abreview

    • ab32572 at 1/200 staining mouse small intestine tissue sections by IHC-P.

      The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the primary antibody. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

      See Abreview

    • ab32572 staining beta Catenin in mouse liver tissue section by Immunohistochemistry (Frozen sections).

      Tissue samples were fixed with formaldehyde and blocked with 5% serum at 40C for 30 minutes. The sample was incubated with primary antibody (1/200) in dilution buffer containing PBS and 3% goat serum at 40C for 9 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.

      See Abreview

    • ab32572 staining human renal carcinoma tissue sections by IHC-P. 

      Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking with 1% milk for 45 minutes at 22°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 22°C.  An HRP conjugated goat anti-rabbit antibody, diluted 1/400, was used as the secondary.

      See Abreview

    文献

    This product has been referenced in:
    • Zhu L  et al. TIPE2 suppresses progression and tumorigenesis of esophageal carcinoma via inhibition of the Wnt/ß-catenin pathway. J Transl Med 16:7 (2018). Read more (PubMed: 29343267) »
    • Xin X  et al. The suppressive role of calcium sensing receptor in endometrial cancer. Sci Rep 8:1076 (2018). Read more (PubMed: 29348629) »
    See all 200 Publications for this product

    客户评价及客户问答

    1-10 of 64 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
    Sample
    Mouse Cell (mouse Embryonic stem cell)
    Specification
    mouse Embryonic stem cell
    Permeabilization
    Yes - 0.1% triton X
    Fixative
    Methanol:acetone

    Abcam user community

    Verified customer

    提交于 Jul 08 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA+Goat IgG as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample
    Human Cell (Breast epithelial)
    Specification
    Breast epithelial
    Permeabilization
    Yes - Triton X-100
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    提交于 Jun 04 2014

    Application
    IHC - Wholemount
    Sample
    Chicken Embryo (Whole embryo - stage HH9-10)
    Specification
    Whole embryo - stage HH9-10

    Ana Azambuja

    Verified customer

    提交于 May 11 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin

    Abcam user community

    Verified customer

    提交于 Feb 26 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin

    Abcam user community

    Verified customer

    提交于 Feb 19 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    tumor xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin

    Abcam user community

    Verified customer

    提交于 Feb 19 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    stomach
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin

    Abcam user community

    Verified customer

    提交于 Feb 19 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EnVision FLEX Target Retrieval Solution
    Permeabilization
    No
    Specification
    Lung
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    提交于 Jan 18 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (foreskin)
    Permeabilization
    No
    Specification
    foreskin
    Blocking step
    BSA as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative
    none

    Mr. Julien Coutier

    Verified customer

    提交于 Jul 05 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Keratinocyte (HaCaT))
    Permeabilization
    Yes - 0.2% Triton x100
    Specification
    Keratinocyte (HaCaT)
    Fixative
    Paraformaldehyde

    Dr. Ann Wheeler

    Verified customer

    提交于 May 17 2017

    1-10 of 64 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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