Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SW1353 treated with rhWnt3A for 24 hours)
Specification
SW1353 treated with rhWnt3A for 24 hours
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Nucleus counter-stain with DAPI
A.) no treatment for SW1353 cells
B.) SW1353 cells treated with 50ng/mL rhWnt3A (from R&D) for 24 hours
A.) no treatment for SW1353 cells
B.) SW1353 cells treated with 50ng/mL rhWnt3A (from R&D) for 24 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 31 2013