为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins

Anti-beta Catenin抗体(ab6302)

  • Datasheet
  • SDS
Reviews (25)Q&A (1)References (170)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
  • Immunocytochemistry - Anti-beta Catenin antibody (ab6302)
  • Western blot - Anti-beta Catenin antibody (ab6302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
  • Western blot - Anti-beta Catenin antibody (ab6302)
  • Immunocytochemistry - Anti-beta Catenin antibody (ab6302)
  • Immunocytochemistry - Anti-beta Catenin antibody (ab6302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
  • Immunocytochemistry - Anti-beta Catenin antibody (ab6302)

Key features and details

  • Rabbit polyclonal to beta Catenin
  • Suitable for: WB, IHC-P, ICC
  • Reacts with: Mouse, Rat, Sheep, Chicken, Human, Pig, Xenopus laevis, Zebrafish, Botryllus schlosseri
  • Isotype: IgG

你可能会感兴趣

生物化学
SAG (Smo agonist), Smoothened agonist (ab142160)
一抗
Product image
Anti-iNOS antibody [EPR16635] (ab178945)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-beta Catenin抗体
    参阅全部 beta Catenin 一抗
  • 描述

    兔多克隆抗体to beta Catenin
  • 宿主

    Rabbit
  • 特异性

    Reacts in dot blot with beta-catenin peptide 768-781 conjugated to BSA. In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with the beta-catenin peptide. Shows no reactivity with BSA conjugated alpha catenin peptide (amino acids 890-901). The antibody does not cross react with a-catenin or ?-catenin (plakoglobin).
  • 经测试应用

    适用于: WB, IHC-P, ICCmore details
  • 种属反应性

    与反应: Mouse, Rat, Sheep, Chicken, Human, Pig, Xenopus laevis, Zebrafish, Botryllus schlosseri
  • 免疫原

    Synthetic peptide:

    PGDSNQLAWFDTDL

    conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse ß Catenin. (Peptide available as ab16377)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.097% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 纯化说明

    Delipidized antiserum.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human beta Catenin protein (Tagged) (ab63175)

应用

Our Abpromise guarantee covers the use of ab6302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/4000. Predicted molecular weight: 85 kDa.
IHC-P Use at an assay dependent concentration.
ICC 1/2000.

靶标

  • 功能

    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 组织特异性

    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • 疾病相关

    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • 序列相似性

    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • 翻译后修饰

    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Target information above from: UniProt accession P35222 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 395964 Chicken
    • Entrez Gene: 1499 Human
    • Entrez Gene: 12387 Mouse
    • Entrez Gene: 397657 Pig
    • Entrez Gene: 84353 Rat
    • Entrez Gene: Sheep
    • Entrez Gene: 30265 Zebrafish
    • Omim: 116806 Human
    • SwissProt: P35222 Human
    • SwissProt: Q02248 Mouse
    • SwissProt: Q9WU82 Rat
    • SwissProt: P26233 Xenopus laevis
    • Unigene: 476018 Human
    • Unigene: 291928 Mouse
    • Unigene: 112601 Rat
    see all
  • 别名

    • b-catenin antibody
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling beta Catenin with ab6302 at 1/20,000 dilution.

     

  • Immunocytochemistry - Anti-beta Catenin antibody (ab6302)
    Immunocytochemistry - Anti-beta Catenin antibody (ab6302)
    Immunocytochemistry analysis of bovine kidney cells labeling beta Catenin with ab6302 at 1/20,000 dilution. Cells were fixed and permeabilized with Methanol followed by Acetone. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Cells were counterstained with DAPI (blue) to stain nuclei.

     

  • Western blot - Anti-beta Catenin antibody (ab6302)
    Western blot - Anti-beta Catenin antibody (ab6302)
    Anti-beta Catenin antibody (ab6302) at 1/8000 dilution + Bovine kidney tissue lysate

    Predicted band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling beta Catenin with ab6302 at 1/20,000 dilution.

     

  • Western blot - Anti-beta Catenin antibody (ab6302)
    Western blot - Anti-beta Catenin antibody (ab6302)
    All lanes : Anti-beta Catenin antibody (ab6302) at 1/4000 dilution

    Lane 1 : 5ug human lung tumour lysate.
    Lane 2 : 10ug human lung tumour lysate.
    Lane 3 : 20ug human lung tumour lysate.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H&L)HRP

    Performed under reducing conditions.

    Predicted band size: 85 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This image is courtesy of an Abreview submitted by Mike Campa on 4 April 2006.

    See Abreview

  • Immunocytochemistry - Anti-beta Catenin antibody (ab6302)
    Immunocytochemistry - Anti-beta Catenin antibody (ab6302)This image is courtesy of an Abreview submitted by Jennifer Schnabel

    ab6302 at 1/2000 dilution staining beta Catenin in human HeLa cells by immunocytochemistry/ immunofluorescence. Sections were paraformaldehyde fixed, permeabilized in 0.3% Triton X prior to blocking in 5% BSA for 2 hours at 27°C and then incubated with ab6302 for 8 hour at 4°C. Alexa fluor® 488 goat polyclonal, diluted 1/1000, was used as the secondary antibody. Counterstaining with DAPI.

    See Abreview

  • Immunocytochemistry - Anti-beta Catenin antibody (ab6302)
    Immunocytochemistry - Anti-beta Catenin antibody (ab6302)This image is courtesy of an Abreview submitted by Roderick Benson

    ab6302 at 1/500 dilution staining beta Catenin in human breast cells by immunocytochemistry/ immunofluorescence. Sections were formaldehyde fixed, permeabilized in 0.5% digitonin prior to incubating with ab6302 for 1 hour. Alexa fluor® 488 goat polyclonal, diluted 1/500, was used as the secondary antibody. Treated samples recieved 10um GSK-3 Inhibitor X.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab6302)This image is courtesy of an anonymous Abreview.

    Immunohistochemical staining of paraffin embedded, PFA fixed, mouse liver using ab6302 at 1/200. Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6, and the sample was permeabilized with 0.05% Triton X 100. The sample was blocked in 5% BSA for 1 hour at room temperature and was then incubated with primary antibody for 16 hours in PBS/0.05% Triton X 100 at 4 °C. An Alexa Fluor® 488 donkey anyti-rabbit was used as the secondary at 1/250.

    See Abreview

  • Immunocytochemistry - Anti-beta Catenin antibody (ab6302)
    Immunocytochemistry - Anti-beta Catenin antibody (ab6302)

    This picture was kindly supplied as part of the review submitted by Mohaiza Dashwood. Immunofluorescence of H29 cells stained with DAPI (blue) and rabbit polyclonal anti-beta-catenin (ab6302), 1/2000) with Alexa Fluor 488 (green) from Molecular Probes.

     

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (170)

    发表研究结果有使用 ab6302?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6302 被引用在 170 文献中.

    • Jamal MH  et al. Rapamycin treatment correlates changes in primary cilia expression with cell cycle regulation in epithelial cells. Biochem Pharmacol 178:114056 (2020). PubMed: 32470549
    • Huang L  et al. Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/ß-catenin axis. J Exp Clin Cancer Res 39:63 (2020). PubMed: 32293515
    • Wu KY  et al. Ependymal Vps35 Promotes Ependymal Cell Differentiation and Survival, Suppresses Microglial Activation, and Prevents Neonatal Hydrocephalus. J Neurosci 40:3862-3879 (2020). PubMed: 32291328
    • Xie WY  et al. Investigation of feather follicle morphogenesis and the expression of the Wnt/ß-catenin signaling pathway in yellow-feathered broiler chick embryos. Br Poult Sci N/A:1-9 (2020). PubMed: 32329625
    • Huo LW  et al. circKIF4A promotes tumorogenesis of glioma by targeting miR-139-3p to activate Wnt5a signaling. Mol Med 26:29 (2020). PubMed: 32268875
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 26 Abreviews or Q&A

    Western blot abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    10 µg
    Specification
    HCT116
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 24 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (cartilage)
    Permeabilization
    Yes - Triton X
    Specification
    cartilage
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Tina Yuan

    Verified customer

    提交于 Jan 04 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (RLE-6TN)
    Permeabilization
    Yes - 0.1% triton
    Specification
    RLE-6TN
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Yuchin Teng

    Verified customer

    提交于 Aug 29 2016

    Question

    What is concentration of product Anti-beta Catenin antibody (ab6302)?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 28 2015

    Answer

    Product Anti-beta Catenin antibody (ab6302) is provided in whole antiserum format, comprised of IgGs as well as other proteins. As this is not a purified format, an exact concentration of antigen-specific IgGs is not available.

    The total IgG concentration in whole antiserum is between 1-10 mg/mL; the antigen-specific IgG concentration in whole antiserum is generally less than 0.5 mg/mL.

    Read More

    Abcam Scientific Support

    回复于 Oct 28 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 4°C
    Sample
    Human Cell (Human arterial myocytes)
    Specification
    Human arterial myocytes
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 08 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
    Sample
    Mouse Tissue sections (Liver, P5)
    Specification
    Liver, P5
    Permeabilization
    Yes - Triton 0,05%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 29 2014

    IHC - Wholemount

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Whole mount 3, 4 and 5 day old larvae)
    Specification
    Whole mount 3, 4 and 5 day old larvae
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 06 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (Sheep tibial plateau articular cartilage and bone)
    Specification
    Sheep tibial plateau articular cartilage and bone
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    DAKO protein block-serum free (X0909) as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 31 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (SW1353 treated with rhWnt3A for 24 hours)
    Specification
    SW1353 treated with rhWnt3A for 24 hours
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 31 2013

    Western blot abreview for Anti-beta Catenin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (SW1353)
    Loading amount
    20 µg
    Specification
    SW1353
    Treatment
    50ng/mL rhWnt3A
    Gel Running Conditions
    Reduced Denaturing (10% Bis-Tris Gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 30 2013

    1-10 of 26 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 3
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    ?ICP?14046373?-1

    ????? 31011502007413?

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有