Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,5% Triton X-100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: AffiniPure Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy2 ®
Dilution
1/100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 08 2010