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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
使用敲除细胞株进行验证

Anti-beta Catenin抗体[15B8] (ab6301)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (40)

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Western blot - Anti-beta Catenin antibody [15B8] (ab6301)
  • Western blot - Anti-beta Catenin antibody [15B8] (ab6301)

Key features and details

  • Mouse monoclonal [15B8] to beta Catenin
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-beta Catenin抗体[15B8]
    参阅全部 beta Catenin 一抗
  • 描述

    小鼠单克隆抗体[15B8] to beta Catenin
  • 宿主

    Mouse
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Zebrafish
  • 免疫原

    Recombinant full length protein corresponding to Chicken beta Catenin.

  • 阳性对照

    • WB: Hap1, HeLa, A43, HEK293, Caco2, NIH3T3 and PC12 cell lysates.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    15B8
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Catenins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

相关产品

  • Alternative Versions

    • Anti-beta Catenin antibody [5H10] (ab231305)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • KO cell lines

    • Human CTNNB1 (beta Catenin) knockout HeLa cell line (ab255352)
  • KO cell lysates

    • Human CTNNB1 (beta Catenin) knockout HeLa cell lysate (ab263756)
  • KO cell pellets

    • Human CTNNB1 (beta Catenin) knockout HeLa cell pellet (ab278960)
  • Recombinant Protein

    • Recombinant Human beta Catenin protein (Tagged) (ab63175)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6301于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 85 kDa).
说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 85 kDa).

靶标

  • 功能

    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 组织特异性

    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • 疾病相关

    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • 序列相似性

    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • 翻译后修饰

    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Target information above from: UniProt accession P35222 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 539003 Cow
    • Entrez Gene: 477032 Dog
    • Entrez Gene: 1499 Human
    • Entrez Gene: 12387 Mouse
    • Entrez Gene: 84353 Rat
    • Entrez Gene: 30265 Zebrafish
    • Omim: 116806 Human
    • SwissProt: Q0VCX4 Cow
    • SwissProt: B6V8E6 Dog
    • SwissProt: P35222 Human
    • SwissProt: Q02248 Mouse
    • SwissProt: Q9WU82 Rat
    • Unigene: 476018 Human
    • Unigene: 291928 Mouse
    • Unigene: 112601 Rat
    see all
  • 别名

    • b-catenin antibody
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

图片

  • Western blot - Anti-beta Catenin antibody [15B8] (ab6301)
    Western blot - Anti-beta Catenin antibody [15B8] (ab6301)
    All lanes : Anti-beta Catenin antibody [15B8] (ab6301) at 1 µg/ml

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : CTNNB1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 85 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab6301 observed at 85 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab6301 was shown to specifically react with beta-Catenin in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and beta Catenin knockout samples were subjected to SDS-PAGE. Ab6301 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-beta Catenin antibody [15B8] (ab6301)
    Western blot - Anti-beta Catenin antibody [15B8] (ab6301)
    All lanes : Anti-beta Catenin antibody [15B8] (ab6301) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa
    Observed band size: 95 kDa why is the actual band size different from the predicted?
    Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab6301 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (40)

发表研究结果有使用 ab6301?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6301 被引用在 40 文献中.

  • Gandhi S  et al. A single-plasmid approach for genome editing coupled with long-term lineage analysis in chick embryos. Development 148:N/A (2021). PubMed: 33688075
  • Hu S  et al. Dermal exosomes containing miR-218-5p promote hair regeneration by regulating ß-catenin signaling. Sci Adv 6:eaba1685 (2020). PubMed: 32832660
  • Cavallero S  et al. Exposure of Human Skin Organoids to Low Genotoxic Stress Can Promote Epithelial-to-Mesenchymal Transition in Regenerating Keratinocyte Precursor Cells. Cells 9:N/A (2020). PubMed: 32824646
  • Aragona M  et al. Mechanisms of stretch-mediated skin expansion at single-cell resolution. Nature 584:268-273 (2020). PubMed: 32728211
  • Takko H  et al. ShapeMetrics: A userfriendly pipeline for 3D cell segmentation and spatial tissue analysis. Dev Biol 462:7-19 (2020). PubMed: 32061886
View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-beta Catenin antibody [15B8]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Heart)
Permeabilization
Yes - Triton 0.1%
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Dec 07 2017

Western blot abreview for Anti-beta Catenin antibody [15B8]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Tissue lysate - other (Liver membrane)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
15 µg
Specification
Liver membrane
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Nov 22 2017

Immunohistochemistry (Frozen sections) abreview for Anti-beta Catenin antibody [15B8]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (foreskin)
Permeabilization
No
Specification
foreskin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
none
Read More

Mr. Julien Coutier

Verified customer

提交于 Jul 05 2017

Immunohistochemistry (Frozen sections) abreview for Anti-beta Catenin antibody [15B8]

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (skin)
Permeabilization
Yes - PBST
Specification
skin
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

提交于 Jan 20 2017

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