为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
I was doing biotinylation of surface proteins and then pull down on streptavidin agarose resin. My proteins can be associated with F-actin. Can this antibody recognize F-actin as well?
Asked on Sep 10 2012
Yes, F-actin is an alternative name for beta-actin, therefore the antibody will cross react. You will need to run the pull down under denaturing conditions in order to avoid pulling down actin with your NMDA receptors. A RIPA buffer would be an ideal buffer to use for this.
回复于 Sep 10 2012