Anti-beta Actin抗体[AC-15] (ab6276)
Key features and details
- Mouse monoclonal [AC-15] to beta Actin
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-beta Actin抗体[AC-15]
参阅全部 beta Actin 一抗 -
描述
小鼠单克隆抗体[AC-15] to beta Actin -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Cow, Dog, Human, African green monkey, Chinese hamster
不与反应: Drosophila melanogaster, Dictyostelium discoideum -
免疫原
Synthetic peptide corresponding to beta Actin aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. Slightly modified ß-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly -Ser-Gly-Lys, conjugated to KLH.
Database link: P60709 -
表位
N-terminal of the beta isoform of actin. -
阳性对照
- ICC/IF: SV40LT-SMC cells WB: HAP1, HeLa, Jurkat, A431, HEK-293, COS-7, NIH/3T3, PC-12, Rat2, CHO, MDBK and MDCK cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from hybridoma cell culture. -
克隆
单克隆 -
克隆编号
AC-15 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
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应用
靶标
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功能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
疾病相关
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
序列相似性
Belongs to the actin family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 81822 Rat
- Omim: 102630 Human
- SwissProt: P48975 Chinese hamster
- SwissProt: P60712 Cow
see all -
别名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
图片
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ab6276 staining beta Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab6276 at a working concentration of 5μg/ml and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 647, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor® 488 (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution
Lane 1 : Wild-type HAP1 cell lysate (20 µg)
Lane 2 : Beta actin knockout HAP1 cell lysate (20 µg)
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution
Predicted band size: 42 kDaLane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Beta actin knockout HAP1 cell lysate (20 µg)Lanes 1 and 2: Merged signal (red and green). Green - beta actin, ab6276 observed at 42 kDa. Red - loading control, ab181602 observed at 37 kDa.
Ab6276 was shown to specifically react with beta actin in wild-type HAP1 cells. No band was observed when beta actin knockout samples were used. Wild-type and beta actin knockout samples were subjected to SDS-PAGE. ab6276 (beta actin) and ab181602 (loading control to GAPDH) were diluted 1/5000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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Western Blot of ab6276 (used as loading control) with whole tissue lysate of human grey matter from BA20 (temporal cortex). Ab6276 was diluted 1/50000 and incubated with the sample for 16 hours at 4°C. 5 µg of lysate was loaded onto the gel, which was blocked with 5% milk for 1 hour at 20°C. An Alexa Fluor® 680 conjugated goat anti-mouse antibody, diluted 1/5000, was used as the secondary.
Bands below actin in image are synaptophysin (SYN).
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All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 6 : Rat2 (rat fibroblast cell line) cell lysate
Lane 7 : CHO (chinese hamster ovary cell line) cell lysate
Lane 8 : MDBK (bovine kidney cell line) cell lysate
Lane 9 : MDCK (canine kidney cell line) cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.
Predicted band size: 42 kDa -
All lanes : Anti-beta Actin antibody [AC-15] (ab6276) at 1/5000 dilution
Lane 1 : HeLa nuclear
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor anti mouse at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa -
MDCK cells induced with increasing amounts of doxycycline to control expression of the gene of interest. All cells were normalized for loading with an albumin protein standard assay. Anti-beta actin (ab6276) was used at a concentration of 1:5000 in a milk blocking solution. B-actin blotting confirms the albumin assay in showing that an equal amount of lysate was loaded in each lane.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2211)
ab6276 被引用在 2211 文献中.
- Van der Zwet JCG et al. STAT5 does not drive steroid resistance in T-cell acute lymphoblastic leukemia despite the activation of BCL2 and BCLXL following glucocorticoid treatment. Haematologica 108:732-746 (2023). PubMed: 35734930
- Luo J et al. Down-regulation of hepatic CLOCK by PPARα is involved in inhibition of NAFLD. J Mol Med (Berl) 101:139-149 (2023). PubMed: 36527474
- Berry MR et al. Investigating PSMA differential expression in canine uroepithelial carcinomas to aid disease-based stratification and guide therapeutic selection. BMC Vet Res 18:441 (2022). PubMed: 36539731
- Sahyon HAE et al. Anti-proliferative effects of the combination of Sulfamethoxazole and Quercetin via caspase3 and NFkB gene regulation: an in vitro and in vivo study. Naunyn Schmiedebergs Arch Pharmacol 395:227-246 (2022). PubMed: 34994822
- Tipton AR & Gorbsky GJ More than two populations of microtubules comprise the dynamic mitotic spindle. J Cell Sci 135:N/A (2022). PubMed: 34907446