Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (Biorad Criterion TGX Stain Free Gel, Any kD, Cat. number 5678123)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Drosophila heads)
Specification
Drosophila heads
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA TBST
Dilution
1/4000
Detection
Detection method
ECL
Bands
Specific: 42 kDa
Exposure
30 second(s)
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提交于 May 02 2014