Anti-beta Actin抗体(ab227387)
Key features and details
- Rabbit polyclonal to beta Actin
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-beta Actin抗体
参阅全部 beta Actin 一抗 -
描述
兔多克隆抗体to beta Actin -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human, Drosophila melanogaster
预测可用于: Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Chimpanzee, Rhesus monkey -
免疫原
Recombinant fragment within Human beta Actin (internal sequence). The exact sequence is proprietary.
Database link: P60709 -
阳性对照
- WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts; Mouse and rat liver tissue extracts; Drosophila melanogaster lysate. IP: HEK-293T whole cell extract. IHC-P: Mouse pancreas and cervix tissues; Rat liver tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Hep G2 whole cell lysate (ab166833)
- Mouse liver tissue lysate - total protein (ab29301)
- HeLa whole cell lysate (ab29545)
- A-431 whole cell lysate (ab30132)
- NIH/3T3 whole cell lysate (ab7179)
- C2C12 whole cell lysate (ab7182)
- RAW 264.7 whole cell lysate (ab7187)
- A-431 whole cell lysate (ab7909)
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227387于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 42 kDa.
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IP |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/5000 - 1/20000. Predicted molecular weight: 42 kDa. |
IP
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
疾病相关
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
序列相似性
Belongs to the actin family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 396526 Chicken
- Entrez Gene: 450133 Chimpanzee
- Entrez Gene: 280979 Cow
- Entrez Gene: 403580 Dog
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
see all -
别名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
图片
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Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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0.5% Triton X-100 followed by ice-cold methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab227387 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 6 : C8D30 whole cell extract
Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 9 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Paraffin-embedded rat liver tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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All lanes : Anti-beta Actin antibody (ab227387) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 5 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 2 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Paraffin-embedded mouse cervix tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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Anti-beta Actin antibody (ab227387) at 1/10000 dilution + Drosophila melanogaster lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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All lanes : Anti-beta Actin antibody (ab227387) at 1/15000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with beta Actin shRNA, whole cell extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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beta Actin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab227387. Western blot was performed from the immunoprecipitate using ab227387. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab227387 in HEK-293T whole cell extract.
Lane 3: ab227387 IP in HEK-293T whole cell extract.
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All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Mouse liver tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Rat liver tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (111)
ab227387 被引用在 111 文献中.
- Wang Z et al. Regenerative peripheral nerve interface prevents neuroma formation after peripheral nerve transection. Neural Regen Res 18:814-818 (2023). PubMed: 36204848
- Paul S et al. Centromere defects, chromosome instability, and cGAS-STING activation in systemic sclerosis. Nat Commun 13:7074 (2022). PubMed: 36400785
- Xiao Y et al. Usnic Acid extends healthspan and improves the neurodegeneration diseases via mTOR/PHA-4 signaling pathway in Caenorhabditis elegans. iScience 25:105539 (2022). PubMed: 36425761
- Ogneva IV et al. Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity. Cells 11:N/A (2022). PubMed: 36497128
- Zhang L et al. Positive Feedback Regulation of Poly(ADP-ribose) Polymerase 1 and the DNA-PK Catalytic Subunit Affects the Sensitivity of Nasopharyngeal Carcinoma to Etoposide. ACS Omega 7:2571-2582 (2022). PubMed: 35097256