Anti-beta III Tubulin (phospho S172)抗体(ab76286)
Key features and details
- Rabbit polyclonal to beta III Tubulin (phospho S172)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-beta III Tubulin (phospho S172)抗体
参阅全部 beta III Tubulin 一抗 -
描述
兔多克隆抗体to beta III Tubulin (phospho S172) -
宿主
Rabbit -
特异性
The ab76286 sequence is identical to similar regions in bI, bII, and bIII tubulin isotypes. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: a wide range of other species -
免疫原
Synthetic peptide corresponding to Human beta III Tubulin (phospho S172) conjugated to keyhole limpet haemocyanin.
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阳性对照
- C2C12 cells; purified brain tubulin treated with ERK2 kinase.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab76286 was cross adsorbed to unphosphorylated beta III Tubulin (Ser 172) peptide before affinity purification using phospho beta III Tubulin (Ser 172) peptide (without carrier). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 38 kDa.
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说明 |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 38 kDa. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
组织特异性
Expression is primarily restricted to central and peripheral nervous system. -
疾病相关
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
序列相似性
Belongs to the tubulin family. -
结构域
The highly acidic C-terminal region may bind cations such as calcium. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 10381 Human
- Entrez Gene: 22152 Mouse
- Omim: 602661 Human
- SwissProt: Q13509 Human
- SwissProt: Q9ERD7 Mouse
- Unigene: 511743 Human
- Unigene: 40068 Mouse
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别名
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
see all
图片
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ab76286, at a 1/100 dilution, staining beta III Tubulin in C2C12 cells by Immunoflurescence.
Image 1: untreated.
Image 2: in the presence of the phospho peptide. -
All lanes : Anti-beta III Tubulin (phospho S172) antibody (ab76286) at 1/1000 dilution
Lane 1 : Purified human brain tubulin, untreated
Lane 2 : Purified human brain tubulin, treated with ERK2 kinase to phosphorylate
Ser 172
Predicted band size: 38 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab76286 被引用在 4 文献中.
- Ge Z et al. The phosphatase of regenerating liver-3 protein (PRL-3) promotes glioma cell invasiveness by interacting with β3 -tubulin. Bioengineered 13:4112-4121 (2022). PubMed: 35098869
- Chang JH et al. Generation of Functional Dopaminergic Neurons from Reprogramming Fibroblasts by Nonviral-based Mesoporous Silica Nanoparticles. Sci Rep 8:11 (2018). PubMed: 29311646
- Ori-McKenney KM et al. Phosphorylation of ß-Tubulin by the Down Syndrome Kinase, Minibrain/DYRK1a, Regulates Microtubule Dynamics and Dendrite Morphogenesis. Neuron 90:551-63 (2016). PubMed: 27112495
- Li WF et al. WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS One 9:e94751 (2014). PubMed: 24728101