Application
ELISA
Sample
Human Cell (H407 cells)
Specification
H407 cells
Blocking step
AbCam ICE kit as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 37°C
Type
Indirect
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: from ICE kit
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Senescence of H407 was induced by Toxin up to 7 days. Beta-galactosidase activity was detected by X-Gal based staining kit. But this antibody does not detect beta-galactosidase on IF and ICE.
Abcam response
Thank you so much for testing this antibody in human.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work in this species. The customer received a discount on a future antibody purchase for testing this product in human. We would welcome additional feedback from other customers as well.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work in this species. The customer received a discount on a future antibody purchase for testing this product in human. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 10 2012