Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12, Muscle and Ovary)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
C2C12, Muscle and Ovary
Blocking step
Sea Block Blocking Agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Seablock Blocking Agent +0.2% Tween
Dilution
1/200
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye Goat Anti Rabbit 800 CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Infrared
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 85 kDa
Additional data
Additional Notes
I would suggest using this antibody in the range 1:200 - 1:500. Higher dilutions might reduce the signal intensity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Francesco Elia Marino
Verified customer
提交于 Sep 23 2016