Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey Blocking Buffer
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Licor Odyssey Clx
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
Positive control
HEK cells, mouse liver
Additional data
Additional Notes
Lane 1: HEK cells
Lane 2: Mouse liver
Lane 3: Mouse retina
Lane 4: Zebrafish retina
Bands seen in lanes 2+3 between 100 and 150 kDa are artifacts resulting from secondary antibody
PVDF membrane
Lysis buffer: RIPA with mixed phosphatase and protease inhibitors
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 03 2017