Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: No buffer: 1mM Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/10000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Very easy to optimise on formalin-fixed pwax sections. The whole brain shows positivity but there are areas/cells that are enriched: the antibody reagent is diluted sufficiently to show this, in the picture of adult mouse brain striatum. Note that the axonal tracks are negatively stained at this dilution factor, yet some cells within those tracks are still strongly positive ( many similar cells outside of the tracks are also positive - I cannot confirm , at this time, what these cells are but they appear to have a wide distribution. The mouse brain was immersion-fixed in 4% Formalin/PBS for 2days, then bisected mid-saggitally. Then further fixed in same fixing solution for a further 3 days. I then processed both halves to pwax on a dip-n-dunk tissue processor using a 20hr-cycle.Sections were cut at 5microns,floated out on water at 47C, mounted on Superfrost Plus slides and dried/melted for 24hrs in a 62C oven.
Our Wolfson Centre for Age-Related Diseases, Kings College London, confirms that this Ab reagent is superb for Western blotting......I just tried it on p.wax sections to see if it worked.
Our Wolfson Centre for Age-Related Diseases, Kings College London, confirms that this Ab reagent is superb for Western blotting......I just tried it on p.wax sections to see if it worked.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Jul 30 2007